Cytokine profiling in human colostrum and milk by protein array
Language English Country Great Britain, England Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
17363422
DOI
10.1373/clinchem.2006.077107
PII: clinchem.2006.077107
Knihovny.cz E-resources
- MeSH
- Time Factors MeSH
- Chemokines analysis MeSH
- Protein Array Analysis MeSH
- Cytokines analysis MeSH
- Adult MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Colostrum chemistry MeSH
- Humans MeSH
- Milk, Human chemistry MeSH
- Intercellular Signaling Peptides and Proteins analysis MeSH
- Postpartum Period MeSH
- Proteomics MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Chemokines MeSH
- Cytokines MeSH
- Intercellular Signaling Peptides and Proteins MeSH
BACKGROUND: Human colostrum and milk contain components that influence development. Our aim was to use a protein array to determine the cytokine profile of human lacteal secretions and changes that occur during the early postpartum period. METHODS: We collected 17 samples of colostrum during the first 2 days postpartum and a 2nd group of 5 sets of 2 to 3 sequential colostrum or milk samples (at 20- to 30-h intervals). We analyzed the samples with array membranes consisting of 42 or 79 antibodies directed against cytokines. RESULTS: In most samples, we detected the previously described cytokines interleukin-8 (IL-8)/CXCL8, epidermal growth factor (EGF), growth-related oncoprotein (GRO)/CXCL1-3, angiogenin, transforming growth factor beta-2, and monocyte chemotactic protein 1 (MCP-1/CCL2). In addition, we found 32 cytokines that have not been described before in colostrum. Cytokine concentrations differed among mothers, and the spectrum of cytokines changed with time after delivery. A significant decrease occurred in IL-12 and macrophage inflammatory protein-1delta/CCL15 and a significant increase in MCP-1/CCL2. The production of angiogenin, vascular endothelial growth factor, GRO/CXCL1-3, EGF, and IL-8/CXCL8 remained high throughout. The concentrations of 2 selected cytokines measured with the array technique and ELISA showed moderate to strong correlation (r = 0.63 for EGF and r = 0.84 for IL-8/CXCL8). CONCLUSION: Despite the lack of precise quantification, the protein array might be suitable for cytokine screening. It allows simultaneous detection of a broad spectrum of cytokines (including those not described before) in lacteal secretions.
References provided by Crossref.org
Colostrum of healthy mothers contains broad spectrum of secretory IgA autoantibodies
Role of intestinal bacteria in gliadin-induced changes in intestinal mucosa: study in germ-free rats
Beneficial health effects of milk and fermented dairy products--review
Perinatal period cytokines related to increased risk of future allergy development
