Proteins of insoluble matrix of avian (gallus gallus) eggshell
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17364661
DOI
10.1080/03008200601003116
PII: 770465817
Knihovny.cz E-resources
- MeSH
- Amino Acids analysis MeSH
- Edetic Acid MeSH
- Electrophoresis, Capillary MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Spectrometry, Mass, Electrospray Ionization MeSH
- Chickens MeSH
- Protoporphyrins analysis MeSH
- Solubility MeSH
- Tandem Mass Spectrometry MeSH
- Egg Shell chemistry MeSH
- Egg Proteins analysis isolation & purification MeSH
- Chromatography, High Pressure Liquid MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amino Acids MeSH
- Edetic Acid MeSH
- ovocalyxin 32 MeSH Browser
- ovocleidin-116 MeSH Browser
- Protoporphyrins MeSH
- protoporphyrin IX MeSH Browser
- Egg Proteins MeSH
The protein composition of the insoluble avian eggshell matrix was studied. The determination of these proteins insoluble in water (EDTA-insoluble) was carried out using enzymatic cleavage followed by a high-performance liquid chromatography-mass spectrometry method. The influence of various enzymes on the protein splitting also was studied. The distribution of proteins depends on the type of layer (localization within the eggshell): ovocalyxin-32 was found mainly in the outer layer (the cuticle); ovocleidin-116 and 17 and ovocalyxin-36 were found throughout the whole eggshell, whereas ovalbumin was only found in the inner layer, the mammillary. The pigment (protoporphyrin IX) was mainly found in the cuticle and is incorporated into the protein network.
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