Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17369037
DOI
10.1016/j.biortech.2007.01.019
PII: S0960-8524(07)00088-0
Knihovny.cz E-resources
- MeSH
- Anthraquinones metabolism MeSH
- Azo Compounds metabolism MeSH
- Coloring Agents metabolism MeSH
- Basidiomycota drug effects enzymology MeSH
- Enzyme Inhibitors pharmacology MeSH
- Hydrogen-Ion Concentration drug effects MeSH
- Culture Media MeSH
- Laccase antagonists & inhibitors isolation & purification metabolism MeSH
- Mycelium drug effects enzymology MeSH
- Oxidation-Reduction drug effects MeSH
- Substrate Specificity drug effects MeSH
- Temperature MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anthraquinones MeSH
- Azo Compounds MeSH
- Coloring Agents MeSH
- Enzyme Inhibitors MeSH
- Culture Media MeSH
- Laccase MeSH
- reactive orange 16 MeSH Browser
- Remazol Brilliant Blue R MeSH Browser
The white rot fungus Irpex lacteus is able to decolorize such synthetic dyes as Reactive Orange 16 and Remazol Brilliant Blue R. Here, we demonstrate that this type of dye decolorization is mainly related to a laccase-like enzyme activity associated with fungal mycelium. In its bound form, the enzyme detected showed a pH optimum of 3.0 for the oxidation of ABTS, DMP and guaiacol, and a pH of 7.0 for syringaldazine. The highest enzymatic activity was obtained with ABTS as substrate. Enzyme activity was fully inhibited with 50mM NaN(3). Depending on the chemical structure of dyes, redox mediators had a positive effect on the dye decolorization by fungal mycelium. Enzyme isolated from fungal mycelium was able to decolorize synthetic dyes in vitro.
References provided by Crossref.org
Irpex lacteus, a white-rot fungus with biotechnological potential--review
