Characterization of a highly pathogenic Bacillus thuringiensis strain isolated from common cockchafer, Melolontha melolontha
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
17575913
DOI
10.1007/bf02932153
Knihovny.cz E-zdroje
- MeSH
- Bacillus thuringiensis klasifikace izolace a purifikace fyziologie MeSH
- bakteriální geny MeSH
- bakteriální proteiny chemie genetika izolace a purifikace MeSH
- bakteriální toxiny chemie genetika izolace a purifikace MeSH
- biologická kontrola škůdců * MeSH
- brouci mikrobiologie MeSH
- buněčná inkluze ultrastruktura MeSH
- druhová specificita MeSH
- endotoxiny chemie genetika izolace a purifikace MeSH
- hemolyziny chemie genetika izolace a purifikace MeSH
- molekulová hmotnost MeSH
- toxiny Bacillus thuringensis MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bakteriální proteiny MeSH
- bakteriální toxiny MeSH
- endotoxiny MeSH
- hemolyziny MeSH
- insecticidal crystal protein, Bacillus Thuringiensis MeSH Prohlížeč
- toxiny Bacillus thuringensis MeSH
A bacterial isolate (Mm2) of Melolontha melolontha was identified and characterized. Based on various morphological, physiological, biochemical and molecular characteristics, it was identified as Bacillus thuringiensis subsp. tenebrionis. This isolate was compared to the reference strains by electron microscopy, SDS-PAGE analysis, plasmid pattern, cry gene content and insecticidal activity. Cells of the isolate harbored flat square inclusions containing a protein component of approximately equal to65 kDa. After trypsin digestion of solubilized crystals, SDS-PAGE resolved a unique proteinase-resistant peptide of approximately equal to 50 kDa. Plasmid pattern showed similar bands to those of the reference strain, PCR analysis showed that the isolate has cry3 gene. Toxicity tests (against 5 coleopteran species) showed 80 % insecticidal activity against the larvae of M. melolontha. The isolate Mm2 may be valuable as biological control agent for M. melolontha and other coleopteran insects.
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