Characterization of a highly pathogenic Bacillus thuringiensis strain isolated from common cockchafer, Melolontha melolontha
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17575913
DOI
10.1007/bf02932153
Knihovny.cz E-resources
- MeSH
- Bacillus thuringiensis classification isolation & purification physiology MeSH
- Genes, Bacterial MeSH
- Bacterial Proteins chemistry genetics isolation & purification MeSH
- Bacterial Toxins chemistry genetics isolation & purification MeSH
- Pest Control, Biological * MeSH
- Coleoptera microbiology MeSH
- Inclusion Bodies ultrastructure MeSH
- Species Specificity MeSH
- Endotoxins chemistry genetics isolation & purification MeSH
- Hemolysin Proteins chemistry genetics isolation & purification MeSH
- Molecular Weight MeSH
- Bacillus thuringiensis Toxins MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
- Bacterial Toxins MeSH
- Endotoxins MeSH
- Hemolysin Proteins MeSH
- insecticidal crystal protein, Bacillus Thuringiensis MeSH Browser
- Bacillus thuringiensis Toxins MeSH
A bacterial isolate (Mm2) of Melolontha melolontha was identified and characterized. Based on various morphological, physiological, biochemical and molecular characteristics, it was identified as Bacillus thuringiensis subsp. tenebrionis. This isolate was compared to the reference strains by electron microscopy, SDS-PAGE analysis, plasmid pattern, cry gene content and insecticidal activity. Cells of the isolate harbored flat square inclusions containing a protein component of approximately equal to65 kDa. After trypsin digestion of solubilized crystals, SDS-PAGE resolved a unique proteinase-resistant peptide of approximately equal to 50 kDa. Plasmid pattern showed similar bands to those of the reference strain, PCR analysis showed that the isolate has cry3 gene. Toxicity tests (against 5 coleopteran species) showed 80 % insecticidal activity against the larvae of M. melolontha. The isolate Mm2 may be valuable as biological control agent for M. melolontha and other coleopteran insects.
See more in PubMed
J Bacteriol. 1983 Apr;154(1):419-28 PubMed
J Appl Microbiol. 1997 Feb;82(2):253-8 PubMed
Curr Microbiol. 2000 Jan;40(1):17-22 PubMed
Plasmid. 1981 May;5(3):352-65 PubMed
Biochem J. 1990 Apr 15;267(2):309-15 PubMed
Gene. 1986;42(1):69-77 PubMed
Microbiol Rev. 1989 Jun;53(2):242-55 PubMed
Nature. 1970 Aug 15;227(5259):680-5 PubMed
Appl Environ Microbiol. 1997 Dec;63(12):4883-90 PubMed
