Off-targeting and other non-specific effects of RNAi experiments in mammalian cells
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem, přehledy
PubMed
17608023
Knihovny.cz E-zdroje
- MeSH
- biologické modely MeSH
- interferony genetika imunologie metabolismus MeSH
- lidé MeSH
- malá interferující RNA genetika MeSH
- RNA interference * MeSH
- signální transdukce genetika fyziologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- Názvy látek
- interferony MeSH
- malá interferující RNA MeSH
The RNA interference (RNAi) approach is an efficient and widely used method for silencing plant and animal genes. However, numerous studies have highlighted several obstacles that need to be overcome in order to achieve a specific and efficient silencing effect. Among the major problems of using RNAi in mammalian systems are non-specific effects, such as an undesirable activation of the IFN response, and off-target effects, whereby partial complementarity of an RNAi trigger to non-targeted transcripts causes unwanted silencing effects. The causes of non-specific effects and possible approaches to minimize them are discussed in this review.
Key Mechanistic Principles and Considerations Concerning RNA Interference
dsRNA expression in the mouse elicits RNAi in oocytes and low adenosine deamination in somatic cells
Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes
