p73 expression in medulloblastoma: TAp73/DeltaNp73 transcript detection and possible association of p73alpha/DeltaNp73 immunoreactivity with survival
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- dítě MeSH
- DNA vazebné proteiny genetika imunologie metabolismus MeSH
- dospělí MeSH
- imunohistochemie MeSH
- jaderné proteiny genetika imunologie metabolismus MeSH
- lidé MeSH
- meduloblastom genetika metabolismus patofyziologie MeSH
- messenger RNA analýza metabolismus MeSH
- míra přežití MeSH
- mladiství MeSH
- mozek metabolismus patologie patofyziologie MeSH
- nádorová transformace buněk genetika metabolismus MeSH
- nádorové biomarkery analýza genetika metabolismus MeSH
- nádorové supresorové proteiny genetika imunologie metabolismus MeSH
- nádory mozku genetika metabolismus patofyziologie MeSH
- předškolní dítě MeSH
- prognóza MeSH
- protein - isoformy genetika izolace a purifikace metabolismus MeSH
- regulace genové exprese u nádorů genetika MeSH
- retrospektivní studie MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA vazebné proteiny MeSH
- jaderné proteiny MeSH
- messenger RNA MeSH
- nádorové biomarkery MeSH
- nádorové supresorové proteiny MeSH
- protein - isoformy MeSH
The human p73 protein is essential for normal morphogenesis and maintenance of neural tissue. Recently, several TP73 transcripts have been revealed in medulloblastoma (MB), the most common malignant brain tumor in children. Here, we performed immunohistochemical analysis on 29 MB specimens using anti-p73alpha and anti-DeltaNp73 antibodies. Real-time PCR quantification was performed to assess TAp73 and DeltaNp73 transcripts in a subset of 13 MB samples. Normal cerebellar tissues and RNA were used for comparison. Pilot clinical-pathological correlations were also provided. We report significant differences for TAp73 and DeltaNp73 mRNA expression between tumor tissues and reference (P = 0.013, P = 0.028). Immunohistochemically, 52 and 29% MB samples were positive for p73alpha and DeltaNp73, respectively. p73alpha expression was found to be in both the nucleus and cytoplasm, whereas DeltaNp73 was localized predominantly in the cytoplasm. In normal cerebellum, positive staining for p73alpha and DeltaNp73 was observed in the Purkinje cells of newborns, not adult samples, which supports the developmental role of TP73 during organogenesis of the human cerebellum. Survival analysis has shown negative relationship of DeltaNp73-immunoreactivity with overall survival (OS) and event free survival (EFS) (P = 0.026 and P = 0.127, respectively). For p73alpha-positive cases, the negative trend in OS (P = 0.149) and EFS (P = 0.216) was also apparent. Our results indicate the involvement of p73 protein in MB tumorigenesis and define TP73 as a potential prognostic and therapeutic target for medulloblastoma.
Acta Neuropathol. 2008 Nov;116(5):579-80 PubMed
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