Hair follicles are unique organs undergoing regular cycles of proliferation, differentiation, and apoptosis. The final step of apoptosis is, in general, mediated by executioner caspases comprising caspase-3, -6 and -7. Despite their commonly accepted apoptotic function, executioner caspases also participate in non-apoptotic processes. In the present study, we investigated activation (cleavage) of caspase-7 in mouse hair follicles and surrounding tissue during embryonic development into adulthood. Casp7 (-/-) mice were examined to understand the effect of caspase-7 deficiency in the skin. The activated form of caspase-7 was observed during embryonic hair follicle development, as well as in the first hair cycle. In general, activation of caspase-7 did not correlate with apoptosis and activation of caspase-3, except during physiological hair follicle regression. Notably, cleaved caspase-7 was observed in mast cells and its deficiency in the adult skin resulted in increased mast cell number. Our study shows for the first time activated caspase-7 in hair follicles and mast cells and indicates its non-apoptotic roles in the skin.

• MeSH
• aktivace enzymů MeSH
• apoptóza * MeSH
• exprese genu MeSH
• kaspasa 3 metabolismus   MeSH
• kaspasa 7 nedostatek   genetika   metabolismus   MeSH
• kaspasy genetika   metabolismus   MeSH
• kůže embryologie   metabolismus   MeSH
• mastocyty metabolismus   MeSH
• myši knockoutované MeSH
• myši MeSH
• počet buněk MeSH
• transport proteinů MeSH
• vlasový folikul embryologie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Three ABC transporters (MDR1, MRP1, BCRP), belonging to the family of multidrug resistance (MDR) proteins, play a crucial role in the protection mechanisms during embryogenesis and mediate drug resistance in cancer cells. The distribution of these transporters in the series of human embryonal/fetal intestine, liver and kidneys of various stages of intrauterine development (IUD) by indirect two-step immunohistochemical method was investigated. The organ- and age-specific expression patterns of these transporters were depicted and compared with the expression in adult organs. The evaluation of intestine and liver samples demonstrate differences in expression pattern of ABC transporters during IUD. On the contrary, in kidneys the age-specific localization was not observed. However, the increasing positivity from the kidney surface towards deeper, more differentiated parts was found. Hopefully, our study may contribute to elucidation of the role of multidrug resistance (MDR) pathways during IUD in man.

• MeSH
• ABC transportéry biosyntéza   genetika   metabolismus   MeSH
• embryonální vývoj genetika   fyziologie   MeSH
• exprese genu MeSH
• játra embryologie   MeSH
• ledviny embryologie   MeSH
• lidé MeSH
• mnohočetná léková rezistence MeSH
• P-glykoproteiny biosyntéza   genetika   metabolismus   MeSH
• střeva embryologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The protein homologous to the tumor suppressor p53, p73, has essential roles in development and tumorigenesis. This protein exists in a wide range of isoforms with different, even antagonistic, functions. However, there are virtually no detailed morphological studies analyzing the endogenous expression of p73 isoforms at the cellular level in cancer cells. In this study, we investigated the expression and subcellular distribution of two N-terminal isoforms, TAp73 and ΔNp73, in medulloblastoma cells using immunofluorescence microscopy. Both proteins were observed in all cell lines examined, but differences were noted in their intracellular localization between the reference Daoy cell line and four newly established medulloblastoma cell lines (MBL-03, MBL-06, MBL-07 and MBL-10). In the new cell lines, TAp73 and ΔNp73 were located predominantly in cell nuclei. However, there was heterogeneity in TAp73 distribution in the cells of all MBL cell lines, with the protein located in the nucleus and also in a limited non-random area in the cytoplasm. In a small percentage of cells, we detected cytoplasmic localization of TAp73 only, i.e., nuclear exclusion was observed. Our results provide a basis for future studies on the causes and function of distinct intracellular localization of p73 protein isoforms with respect to different protein-protein interactions in medulloblastoma cells.

• MeSH
• buněčné jádro metabolismus   MeSH
• dítě MeSH
• DNA vazebné proteiny chemie   metabolismus   MeSH
• intracelulární prostor metabolismus   MeSH
• jaderné proteiny chemie   metabolismus   MeSH
• lidé MeSH
• meduloblastom metabolismus   patologie   MeSH
• nádorové buněčné linie MeSH
• nádorové supresorové proteiny chemie   metabolismus   MeSH
• předškolní dítě MeSH
• protein - isoformy metabolismus   MeSH
• transport proteinů MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Rosiglitazone, peroxisome proliferator-activated receptor-gamma agonist, is an insulin sensitizing agent in peripheral tissues. This study investigated islet hormones and hormone-like peptides expression patterns in rosiglitazone treated streptozotocin (STZ)-diabetic rats by using immunohistochemistry and in situ hybridization methods. Animals were divided into four groups. I. Group: Intact control rats. II. Group: Rosiglitazone-treated controls. III. Group: STZ-diabetic rats. IV. Group: Rosiglitazone-treated diabetic animals. Rosiglitazone was given for 7 days at a dose of 20 mg/kg body weight. In the STZ-diabetic group, there were significant differences in islet hormones and hormone like peptides cell numbers compared to rosiglitazone control group and intact control group. There were significant differences in cocaine- and amphetamine-regulated transcript (CART) and pancreatic polypeptide (PP) cell numbers between rosiglitazone control group and rosiglitazone + STZ-diabetic group. We detected a significant decrease in glucagon mRNA signals in rosiglitazone-treated control group compared to intact controls. We found a statistically significant difference in islet amyloid polypeptide (IAPP) mRNA signals between the STZ-diabetic group and the rosiglitazone + STZ-diabetic group. Besides, we also demonstrated co-localization of peptides by using double and triple histochemistry. In conclusion, our results show that short-term rosiglitazone treatment had a preservative effect to some extent on the expression of islet hormones and hormone-like peptides to maintain the islet function.

• MeSH
• experimentální diabetes mellitus MeSH
• hormony metabolismus   MeSH
• hybridizace in situ MeSH
• hypoglykemika farmakologie   MeSH
• imunohistochemie MeSH
• krysa rodu rattus MeSH
• Langerhansovy ostrůvky cytologie   metabolismus   účinky léků   MeSH
• peptidy metabolismus   MeSH
• potkani Wistar MeSH
• PPAR gama agonisté   MeSH
• thiazolidindiony farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• práce podpořená grantem MeSH

• MeSH
• buněčná diferenciace MeSH
• finanční podpora výzkumu jako téma MeSH
• galektiny analýza   MeSH
• keratinocyty chemie   MeSH
• lidé MeSH
• vlasový folikul cytologie   chemie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• srovnávací studie MeSH

• MeSH
• aktiny biosyntéza   genetika   MeSH
• finanční podpora výzkumu jako téma MeSH
• kardiomyocyty metabolismus   ultrastruktura   MeSH
• konexin 43 biosyntéza   genetika   MeSH
• krysa rodu rattus MeSH
• kultivované buňky MeSH
• regulace genové exprese účinky záření   MeSH
• trijodthyronin farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH