Femoral bone microstructure in 1-month-old non-transgenic versus transgenic rabbits with the WAP-hFVIII gene construct
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18226360
PII: file/6088/fb2008a0003.pdf
Knihovny.cz E-resources
- MeSH
- Factor VIII genetics MeSH
- Femur cytology MeSH
- Calcification, Physiologic MeSH
- Animals, Genetically Modified MeSH
- Bone Density MeSH
- Rabbits MeSH
- Humans MeSH
- Milk Proteins genetics MeSH
- Animals, Newborn MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Factor VIII MeSH
- Milk Proteins MeSH
- whey acidic proteins MeSH Browser
Differences in microscopic structure of the femur between 1-month-old transgenic rabbits carrying the hFVIII gene and non-transgenic rabbits were investigated. Bone microstructure was evaluated from the point of view of qualitative and quantitative histological characteristics. We identified fibrolamellar bone tissue only in the transgenic animals. Measured values for area, perimeter of the Haversian canals and minimum diameter of the primary osteons' vascular canals were higher in 1-month-old transgenic individuals (P < 0.05; P < 0.001). We also observed lower concentrations of Ca, P, K, solids, and total mineral content in femora of transgenic rabbits. A statistically significant difference was observed for the concentration of Ca (P < 0.05). Our results indicate evident changes in both qualitative and quantitative histological characteristics of the femur, which result especially in better blood supply and slightly reduced mineralization process in 1-month-old transgenic rabbits.
