Nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pA81 from Achromobacter xylosoxidans A8
Language English Country France Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
18249097
DOI
10.1016/j.resmic.2007.11.018
PII: S0923-2508(07)00241-0
Knihovny.cz E-resources
- MeSH
- Achromobacter denitrificans genetics MeSH
- Hydrocarbons, Aromatic metabolism MeSH
- Genes, Bacterial MeSH
- DNA, Bacterial chemistry genetics MeSH
- Evolution, Molecular MeSH
- Molecular Sequence Data MeSH
- Multigene Family MeSH
- Open Reading Frames MeSH
- Mixed Function Oxygenases genetics MeSH
- Oxygenases genetics MeSH
- Plasmids chemistry genetics MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Metals, Heavy metabolism MeSH
- DNA Transposable Elements MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Names of Substances
- Hydrocarbons, Aromatic MeSH
- DNA, Bacterial MeSH
- Mixed Function Oxygenases MeSH
- Oxygenases MeSH
- salicylic acid 5-hydroxylase MeSH Browser
- Metals, Heavy MeSH
- DNA Transposable Elements MeSH
The complete 98,192bp nucleotide sequence was determined for plasmid pA81, which is harbored by the haloaromatic acid-degrading bacterium Achromobacter xylosoxidans A8. The majority of the 103 open reading frames identified on pA81 could be categorized as either "backbone" genes, genes encoding (halo)aromatic compound degradation, or heavy metal resistance determinants. The backbone genes controlled conjugative transfer, replication and plasmid stability, and were well conserved with other IncP1-beta plasmids. Genes encoding (halo)aromatic degradation were clustered within a type I transposon, TnAxI, and included two ring-hydroxylating oxygenases (ortho-halobenzoate oxygenase, salicylate 5-hydroxylase) and a modified ortho-cleavage pathway for chlorocatechol degradation. The cluster of heavy metal resistance determinants was contained within a Type II transposon TnAxII, and included a predicted P-type ATPase and cation diffusion facilitator system. Genes identical to those carried by TnAxI and TnAxII were identified on other biodegradative/resistance plasmids and genomic islands, indicating an evolutionary relationship between these elements. Collectively, these insights further our understanding of how mobile elements, and interactions between mobile elements affect the fate of organic and inorganic toxicants in the environment.
References provided by Crossref.org
Complete genome sequence of the haloaromatic acid-degrading bacterium Achromobacter xylosoxidans A8
GENBANK
AJ875020
