Nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pA81 from Achromobacter xylosoxidans A8
Jazyk angličtina Země Francie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
18249097
DOI
10.1016/j.resmic.2007.11.018
PII: S0923-2508(07)00241-0
Knihovny.cz E-zdroje
- MeSH
- Achromobacter denitrificans genetika MeSH
- aromatické uhlovodíky metabolismus MeSH
- bakteriální geny MeSH
- DNA bakterií chemie genetika MeSH
- molekulární evoluce MeSH
- molekulární sekvence - údaje MeSH
- multigenová rodina MeSH
- otevřené čtecí rámce MeSH
- oxygenasy se smíšenou funkcí genetika MeSH
- oxygenasy genetika MeSH
- plazmidy chemie genetika MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA MeSH
- těžké kovy metabolismus MeSH
- transpozibilní elementy DNA MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Názvy látek
- aromatické uhlovodíky MeSH
- DNA bakterií MeSH
- oxygenasy se smíšenou funkcí MeSH
- oxygenasy MeSH
- salicylic acid 5-hydroxylase MeSH Prohlížeč
- těžké kovy MeSH
- transpozibilní elementy DNA MeSH
The complete 98,192bp nucleotide sequence was determined for plasmid pA81, which is harbored by the haloaromatic acid-degrading bacterium Achromobacter xylosoxidans A8. The majority of the 103 open reading frames identified on pA81 could be categorized as either "backbone" genes, genes encoding (halo)aromatic compound degradation, or heavy metal resistance determinants. The backbone genes controlled conjugative transfer, replication and plasmid stability, and were well conserved with other IncP1-beta plasmids. Genes encoding (halo)aromatic degradation were clustered within a type I transposon, TnAxI, and included two ring-hydroxylating oxygenases (ortho-halobenzoate oxygenase, salicylate 5-hydroxylase) and a modified ortho-cleavage pathway for chlorocatechol degradation. The cluster of heavy metal resistance determinants was contained within a Type II transposon TnAxII, and included a predicted P-type ATPase and cation diffusion facilitator system. Genes identical to those carried by TnAxI and TnAxII were identified on other biodegradative/resistance plasmids and genomic islands, indicating an evolutionary relationship between these elements. Collectively, these insights further our understanding of how mobile elements, and interactions between mobile elements affect the fate of organic and inorganic toxicants in the environment.
Citace poskytuje Crossref.org
Complete genome sequence of the haloaromatic acid-degrading bacterium Achromobacter xylosoxidans A8
GENBANK
AJ875020
