Activation of adenosine A(3) receptors potentiates stimulatory effects of IL-3, SCF, and GM-CSF on mouse granulocyte-macrophage hematopoietic progenitor cells
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18380545
DOI
10.33549/physiolres.931454
PII: 1454
Knihovny.cz E-resources
- MeSH
- Adenosine analogs & derivatives pharmacology MeSH
- Cell Division drug effects MeSH
- Granulocyte-Macrophage Colony-Stimulating Factor pharmacology MeSH
- Granulocytes cytology MeSH
- Hematopoietic Stem Cells cytology drug effects metabolism MeSH
- Hepatocyte Growth Factor pharmacology MeSH
- Mice, Inbred Strains MeSH
- Interleukin-3 pharmacology MeSH
- Cells, Cultured MeSH
- Macrophages cytology MeSH
- Mice MeSH
- Receptor, Adenosine A3 metabolism MeSH
- Drug Synergism MeSH
- In Vitro Techniques MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adenosine MeSH
- Granulocyte-Macrophage Colony-Stimulating Factor MeSH
- Hepatocyte Growth Factor MeSH
- Interleukin-3 MeSH
- N(6)-(3-iodobenzyl)-5'-N-methylcarboxamidoadenosine MeSH Browser
- Receptor, Adenosine A3 MeSH
Adenosine A(3) receptor agonist N(6)-(3-iodobenzyl)adenosine-5'-N-methyluronamide (IB-MECA) has been tested from the point of view of potentiating the effects of hematopoietic growth factors interleukin-3 (IL-3), stem cell factor (SCF), granulocyte-macrophage colony-stimulating factor (GM-CSF), and granulocyte colony-stimulating factor (G-CSF) on the growth of hematopoietic progenitor cells for granulocytes and macrophages (GM-CFC) in suspension of normal mouse bone marrow cells in vitro. IB-MECA alone induced no GM-CFC growth. Significant elevation of numbers of GM-CFC evoked by the combinations of IB-MECA with IL-3, SCF, or GM-CSF as compared with these growth factors alone has been noted. Combination of IB-MECA with G-CSF did not induce significantly higher numbers of GM-CFC in comparison with G-CSF alone. Joint action of three drugs, namely of IB-MECA + IL-3 + GM-CSF, produced significantly higher numbers of GM-CFC in comparison with the combinations of IB-MECA + IL-3, IB-MECA + GM-CSF, or IL-3 + GM-CSF. These results give evidence of a significant role of selective activation of adenosine A(3) receptors in stimulation of the growth of granulocyte/ macrophage hematopoietic progenitor cells.
References provided by Crossref.org
Lack of adenosine A3 receptors causes defects in mouse peripheral blood parameters
