Phytase activity and its regulation in a rhizospheric strain of Serratia plymuthica
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- 6-fytasa chemie genetika metabolismus MeSH
- bakteriální proteiny chemie genetika metabolismus MeSH
- kořeny rostlin mikrobiologie MeSH
- regulace genové exprese enzymů * MeSH
- regulace genové exprese u bakterií * MeSH
- Serratia chemie enzymologie genetika MeSH
- sigma faktor genetika metabolismus MeSH
- stabilita enzymů MeSH
- Vitis mikrobiologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 6-fytasa MeSH
- bakteriální proteiny MeSH
- GacA protein, Bacteria MeSH Prohlížeč
- sigma faktor MeSH
Serratia plymuthica strain IC1270 isolated from the rhizosphere, possessing antagonistic activity towards a wide range of plant-pathogenic fungi, is able to hydrolyze phytate. Phytase activity was found intracellularly, while no activity was detected in the culture liquid. Optimum activity was found at pH 4-5; it completely disappeared at pH > 7.0 and 2.5. Phytase production was practically absent in the exponential phase and reached a maximum in the late stationary phase. Mutations of genes grrA and grrS, encoding GacA/GacS-like 2-component global regulatory system, or in gene rpoS encoding the sigma factor RpoS subunit of RNA polymerase, led to a deficiency in phytase production. Introduction into mutants of the respective wild-type genes cloned into the wide-range plasmid pJFF224-NX under the control of the bacteriophage T4 gene 32 promoter complemented this deficiency. This is the first report implicating the GacA/GacS global regulators and RpoS factor in phytase production in bacteria.
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