Aspergillus niger pH 2.1 optimum acid phosphatase with high affinity for phytate
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
17455790
DOI
10.1007/bf02931618
Knihovny.cz E-zdroje
- MeSH
- Aspergillus niger enzymologie MeSH
- chromatografie iontoměničová MeSH
- gelová chromatografie MeSH
- koncentrace vodíkových iontů MeSH
- kyselá fosfatasa izolace a purifikace metabolismus MeSH
- kyselina fytová metabolismus MeSH
- nitrofenoly metabolismus MeSH
- organofosforové sloučeniny metabolismus MeSH
- stabilita enzymů MeSH
- substrátová specifita MeSH
- teplota MeSH
- ultrafiltrace MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kyselá fosfatasa MeSH
- kyselina fytová MeSH
- nitrofenoly MeSH
- nitrophenylphosphate MeSH Prohlížeč
- organofosforové sloučeniny MeSH
An extracellular acid phosphatase isolated from the culture of a wild strain Aspergillus niger, producing the dephosphorylating 3-phytase, was obtained in a homogeneous form by sequential application of ultrafiltration through PS 50 membrane, gel filtration on Sephadex G-100 and ion exchange chromatography on DEAE-Sepharose CL 6B and CM-Sepharose CL 6B. The enzyme showed a maximum catalytic value in a strongly acidic range (pH 2.0-2.4) with pHopt 2.1 and topt 66 degrees C. The acid phosphatase showed a wide substrate specificity and a high affinity for sodium phytate, 2.5x higher than with 4-nitrophenyl phosphate. This property of the acid phosphatase demonstrated that it is a potent 3-phytase at pH 2.1 and is of great significance for a practical application of the dephosphorylating complex--its addition to the diets of monogastric animals in view of the low pH values in the digestive tract.
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Nature. 1970 Aug 15;227(5259):680-5 PubMed
Anal Biochem. 1981 May 15;113(2):313-7 PubMed
Folia Microbiol (Praha). 1998;43(4):323-38 PubMed
Arch Tierernahr. 1999;52(1):15-27 PubMed
Biosci Biotechnol Biochem. 1997 Sep;61(9):1512-7 PubMed
Appl Environ Microbiol. 1998 Nov;64(11):4446-51 PubMed
Biochem Biophys Res Commun. 1994 Aug 30;203(1):182-9 PubMed
World J Microbiol Biotechnol. 1993 Jan;9(1):117-9 PubMed
J Mol Biol. 1999 May 21;288(5):965-74 PubMed
Prep Biochem. 1987;17(1):63-91 PubMed
Prep Biochem. 1987;17(4):397-422 PubMed
Folia Microbiol (Praha). 2003;48(6):767-70 PubMed
Phytase activity and its regulation in a rhizospheric strain of Serratia plymuthica
Phytase from antarctic yeast strain Cryptococcus laurentii AL27
