Genetic relatedness of Trichoderma isolates antagonistic against Fusarium oxysporum f.sp. dianthi inflicting carnation wilt
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- antibióza * MeSH
- chitinasy genetika metabolismus MeSH
- fungální proteiny genetika metabolismus MeSH
- Fusarium fyziologie MeSH
- fylogeneze MeSH
- genetická variace MeSH
- nemoci rostlin mikrobiologie MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- půdní mikrobiologie * MeSH
- Trichoderma klasifikace genetika izolace a purifikace fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- chitinasy MeSH
- fungální proteiny MeSH
Twenty-eight isolates of Trichoderma belonging to four different species were screened in vitro for their antagonistic ability against Fusarium oxysporum f.sp. dianthi causing carnation wilt. Three different levels of antagonism observed in dual plate assay were further confirmed by cell-free culture filtrate experiments. Isolates showing class I level of antagonism produced maximum lytic enzymes, chitinases and beta-1,3-glucanases. Genetic variability of 25 selected isolates was assessed by random amplified polymorphic DNA technique and the amplified products were correlated for their level of antagonism. Unweighed pair-group method with arithmetical averages cluster analysis revealed prominent inter-and intraspecific genetic variation among the isolates. Based on their genetic relationship, the isolates were mainly distributed into 3 major groups representing T. atroviride, T. pseudokoningii and T. harzianum, with 20-35% interspecific dissimilarity. However, the polymorphism shown by the isolates did not correlate to their level of antagonism.
Zobrazit více v PubMed
Nucleic Acids Res. 1990 Dec 25;18(24):7213-8 PubMed
Appl Environ Microbiol. 1999 Dec;65(12):5421-6 PubMed
Curr Genet. 2001 Jan;38(6):343-50 PubMed
Appl Environ Microbiol. 1994 Dec;60(12):4364-70 PubMed
FEMS Microbiol Lett. 2004 Nov 1;240(1):87-97 PubMed
Curr Microbiol. 2006 Apr;52(4):310-6 PubMed
FEMS Microbiol Lett. 1995 Mar 1;126(3):249-55 PubMed
Appl Environ Microbiol. 1995 Mar;61(3):1004-12 PubMed
Biochim Biophys Acta. 1958 Sep;29(3):522-34 PubMed
J Antibiot (Tokyo). 1994 Feb;47(2):173-82 PubMed
J Clin Microbiol. 1999 Jun;37(6):1661-9 PubMed
Mol Gen Genet. 1997 Sep;256(2):127-35 PubMed
