Distinctive archaebacterial species associated with anaerobic rumen protozoan Entodinium caudatum
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Anaerobiosis MeSH
- Archaea classification genetics growth & development isolation & purification MeSH
- Rumen microbiology parasitology MeSH
- Ciliophora genetics growth & development isolation & purification MeSH
- DNA, Archaeal analysis isolation & purification MeSH
- Species Specificity MeSH
- Phylogeny MeSH
- Culture Media MeSH
- Molecular Sequence Data MeSH
- Sheep MeSH
- DNA, Protozoan analysis isolation & purification MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Symbiosis MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Archaeal MeSH
- Culture Media MeSH
- DNA, Protozoan MeSH
- RNA, Ribosomal, 16S MeSH
The diversity of archaebacteria associated with anaerobic rumen protozoan Entodinium caudatum in long term in vitro culture was investigated by denaturing gradient gel electrophoresis (DGGE) analysis of hypervariable V3 region of archaebacterial 16S rRNA gene. PCR was accomplished directly from DNA extracted from a single protozoal cell and from total community genomic DNA and the obtained fingerprints were compared. The analysis indicated the presence of a solitary intensive band present in Entodinium caudatum single cell DNA, which had no counterparts in the profile from total DNA. The identity of archaebacterium represented by this band was determined by sequence analysis which showed that the sequence fell to the cluster of ciliate symbiotic methanogens identified recently by 16S gene library approach.
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