Differing expression of genes involved in non-transferrin iron transport across plasma membrane in various cell types under iron deficiency and excess
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- buněčná membrána metabolismus MeSH
- buněčné linie MeSH
- ceruloplasmin genetika metabolismus MeSH
- cytochromy typu b genetika metabolismus MeSH
- exprese genu * MeSH
- kultivační média chemie MeSH
- lidé MeSH
- membránové proteiny * genetika metabolismus MeSH
- oxidoreduktasy genetika metabolismus MeSH
- proteiny přenášející kationty genetika metabolismus MeSH
- transferin metabolismus MeSH
- železo metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- ceruloplasmin MeSH
- CYBRD1 protein, human MeSH Prohlížeč
- cytochromy typu b MeSH
- Ferroportin MeSH
- HEPH protein, human MeSH Prohlížeč
- kultivační média MeSH
- membránové proteiny * MeSH
- oxidoreduktasy MeSH
- proteiny přenášející kationty MeSH
- solute carrier family 11- (proton-coupled divalent metal ion transporters), member 2 MeSH Prohlížeč
- transferin MeSH
- železo MeSH
We studied the effect of iron deficiency, i.e., 24-h preincubation in iron-free medium, and the effect of high level of non-transferrin iron, i.e., the preincubation in ferric citrate medium containing 500 microM ferric citrate, on the expression of DMT1, Dcytb, ferroportin, hephaestin, and ceruloplasmin in various functional types of human cells. The expression of these proteins potentially involved in non-transferrin iron transport across cell membranes was tested on mRNA level by quantitative real-time PCR as well as on protein level by western blot analysis in Caco-2 (colorectal carcinoma), K562 (erythroleukemia), and HEP-G2 (hepatocellular carcinoma) cells. We found that changes in non-transferrin iron availability, i.e., iron deficiency and high level of non-transferrin iron, affect the expression of tested proteins in a cell type-specific manner. We also demonstrated that changes in the expression on mRNA level do not often correlate with relevant changes on protein level.
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