Cuticular water permeabilities of adaxial and abaxial leaf surfaces and their dependence on relative air humidity (RH) applied in long-term and short-term regimes have been analysed for Hedera helix, native in a temperate climate, and Zamioculcas zamiifolia, native in subtropical regions. The water permeability of cuticular membranes (CM) isolated from the adaxial (astomatous) and abaxial (stomatous) leaf sides was measured using a method which allowed the separation of water diffusion through the remnants of the original stomatal pores from water diffusion through the solid cuticle. The long-term effects of low (20-40%) or high (60-80%) RH applied during plant growth and leaf ontogeny ('growth RH') and the short-term effects of applying 2% or 100% RH while measuring permeability ('measurement RH') were investigated. With both species, water permeability of the solid stomatous CM was significantly higher than the permeability of the astomatous CM. Adaxial cuticles of plants grown in humid air were more permeable to water than those from dry air. The adaxial CM of the drought-tolerant H. helix was more permeable and more sensitive to growth RH than the adaxial CM of Z. zamiifolia, a species avoiding water stress. However, permeability of the solid abaxial CM was similar in both species and independent of growth RH. The lack of a humidity response in the abaxial CM is attributed to a higher degree of cuticular hydration resulting from stomatal transpiration. The ecophysiological significance of higher permeability of the solid stomatous CM compared to the astomatous CM is discussed.

Faculty of Science University of South Bohemia Branisovská 31 37005 Ceské Budejovice Czech Republic

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Bakker JC. Effects of humidity on stomatal density and its relation to leaf conductance. Scientia Horticulturae. 1991;48:205–212.

Beyer M, Lau S, Knoche M. Studies on water transport through the sweet cherry fruit surface. IX. Comparing permeability in water uptake and transpiration. Planta. 2005;220:474–485. PubMed

Bird SM, Gray JE. Signals from the cuticle affect epidermal cell differentiation. New Phytologist. 2003;157:9–23. PubMed

Burghardt M, Riederer M. Ecophysiological relevance of cuticular transpiration of deciduous and evergreen plants in relation to stomatal closure and leaf water potential. Journal of Experimental Botany. 2003;54:1941–1949. PubMed

Burghardt M, Riederer M. Cuticular transpiration. In: Riederer M, Müller C, editors. Biology of the plant cuticle. Blackwell Publishing; 2006. pp. 292–311.

Burkhardt J, Kaiser H, Goldbach H, Kappen L. Measurements of electrical leaf surface conductance reveal recondensation of transpired water vapour on leaf surfaces. Plant, Cell and Environment. 1999;22:189–196.

Cussler EL. Diffusion. Mass transfer in fluid systems. Cambridge: Cambridge University Press; 1987.

Eamus D, Taylor DT, Macinnis-Ng CMO, Shanahan S, De Silva L. Comparing model predictions and experimental data for the response of stomatal conductance and guard cell turgor to manipulations of cuticular conductance, leaf-to-air vapour pressure difference and temperature: feedback mechanisms are able to account for all observations. Plant, Cell and Environment. 2008;31:269–277. PubMed

Geyer U, Schönherr J. The effect of the environment on the permeability and composition of citrus leaf cuticles. 1. Water permeability of isolated cuticular membranes. Planta. 1990;180:147–153. PubMed

Goodwin SM, Jenks MA. Plant cuticle function as a barrier to water loss. In: Jenks MA, Hasegawa PM, editors. Plant abiotic stress. Blackwell Publishing; 2005. pp. 14–36.

Grivet D, Petit RJ. Phylogeography of the common ivy (Hedera sp.) in Europe: genetic differentiation through space and time. Molecular Ecology. 2002;11:1351–1362. PubMed

Hirai G, Okumura T, Ashida K, Inamura T, Tanaka O, Chujo H, Hirano T. Varietal differences in the morphophysiological response to atmospheric humidity in rice. Plant Production Science. 2002;5:101–109.

Hoad SP, Grace J, Jeffree CE. Humidity response of cuticular conductance of beech (Fagus sylvatica L.) leaf discs maintained at high relative water content. Journal of Experimental Botany. 1997;48:1969–1975.

Holtum JAM, Winter K, Weeks MA, Sexton TR. Crassulacean acid metabolism in the ZZ plant, Zamioculcas zamiifolia (Araceae) American Journal of Botany. 2007;94:1670–1676. PubMed

Kerstiens G. Cuticular water permeability and its physiological significance. Journal of Experimental Botany. 1996a;47:1813–1832.

Kerstiens G. Signalling across the divide: a wider perspective of cuticular structure–function relationships. Trends in Plant Science. 1996b;1:125–129.

Kerstiens G. Water transport in plant cuticles: an update. Journal of Experimental Botany. 2006;57:2493–2499. PubMed

Klooster B, Palmer-Young E. Water stress marginally increases stomatal density in E. canadensis, but not in A. gerardii. Tillers. 2004;5:35–40.

Knoche M, Peschel S, Hinz M, Bukovac MJ. Studies on water transport through the sweet cherry fruit surface: characterizing conductance of the cuticular membrane using pericarp segments. Planta. 2000;212:127–135. PubMed

Larcher W. Physiological plant ecology. Berlin, Heidelberg: Springer-Verlag; 1995.

Metcalfe DJ. Hedera helix L. Journal of Ecology. 2005;93:632–648.

Osborn JM, Taylor TN. Morphological and ultrastructural studies of plant cuticular membranes 1. Sun and shade leaves of Quercus velutina (Fagaceae) Botanical Gazette. 1990;151:465–476.

Pesacreta TC, Hasenstein KH. The internal cuticle of Cirsium horridulum (Asteraceae) leaves. American Journal of Botany. 1999;86:923–928. PubMed

Price CE. A review of the factors influencing the penetration of pesticides through plant leaves. In: Cutler DF, Alvin KL, Price CE, editors. The plant cuticle. London: Academic Press; 1982. pp. 237–252.

Riederer M, Schreiber L. Protecting against water loss: analysis of the barrier properties of plant cuticles. Journal of Experimental Botany. 2001;52:2023–2032. PubMed

Šantrůček J, Schreiber L, Šimáňová E, Šimková M. Permeability of intact plant cuticles measured by chlorophyll fluorescence imaging. Biologické listy. 2000;65:295–298.

Šantrůček J, Šimáňová E, Karbulková J, Šimková M, Schreiber L. A new technique for measurement of water permeability of stomatous cuticular membranes isolated from Hedera helix leaves. Journal of Experimental Botany. 2004;55:1411–1422. PubMed

Schlegel TK, Schönherr J, Schreiber L. Size selectivity of aqueous pores in stomatous cuticles of Vicia faba leaves. Planta. 2005;221:648–655. PubMed

Schlegel TK, Schönherr J, Schreiber L. Rates of foliar penetration of chelated Fe(III): role of light, stomata, species, and leaf age. Journal of Agricultural and Food Chemistry. 2006;54:6809–6813. PubMed

Schönherr J. Calcium chloride penetrates plant cuticles via aqueous pores. Planta. 2000;212:112–118. PubMed

Schönherr J. A mechanistic analysis of penetration of glyphosate salts across astomatous cuticular membranes. Pest Management Science. 2002;58:343–351. PubMed

Schönherr J. Characterization of aqueous pores in plant cuticles and permeation of ionic solutes. Journal of Experimental Botany. 2006;57:2471–2491. PubMed

Schönherr J, Riederer M. Plant cuticles sorb lipophilic compounds during enzymatic isolation. Plant Cell and Environment. 1986;9:459–466.

Schönherr J, Schreiber L. Size selectivity of aqueous pores in astomatous cuticular membranes isolated from Populus canescens (Aiton) Sm. leaves. Planta. 2004;219:405–411. PubMed

Schreiber L. Untersuchungen zur Schadstoffaufnahme in Blätter. PhD thesis, Technische. Germany: Universität München; 1990. pp. 49–50.

Schreiber L. Polar paths of diffusion across plant cuticles: new evidence for an old hypothesis. Annals of Botany. 2005;95:1069–1073. PubMed PMC

Schreiber L. Review of sorption and diffusion of lipophilic molecules in cuticular waxes and the effects of accelerators on solute mobilities. Journal of Experimental Botany. 2006;57:2515–2523. PubMed

Schreiber L, Riederer M. Ecophysiology of cuticular transpiration: comparative investigation of cuticular water permeability of plant species from different habitats. Oecologia. 1996;107:426–432. PubMed

Schreiber L, Skrabs M, Hartmann KD, Diamantopoulos P, Šimáňová E, Šantrůček J. Effect of humidity on cuticular water permeability of isolated cuticular membranes and leaf disks. Planta. 2001;214:274–282. PubMed

Snyder KA, Richards JH, Donovan LA. Night-time conductance in C3 and C4 species: do plants lose water at night? Journal of Experimental Botany. 2003;54:861–865. PubMed

Talbott LD, Rahveh E, Zeiger E. Relative humidity is a key factor in the acclimation of the stomatal response to CO2. Journal of Experimental Botany. 2003;54:2141–2147. PubMed

Torre S, Fjeld T, Gislerod HR, Moe R. Leaf anatomy and stomatal morphology of greenhouse roses grown at moderate or high air humidity. Journal of the American Society for Horticultural Science. 2003;128:598–602.

Van Hove LWA, Adema EH. The effective thickness of water films on leaves. Atmospheric Environment. 1996;30:2933–2936.

Woodward FI. Do plants really need stomata? Journal of Experimental Botany. 1998;49:471–480.

Amphistomy: stomata patterning inferred from 13C content and leaf-side-specific deposition of epicuticular wax

The why and how of sunken stomata: does the behaviour of encrypted stomata and the leaf cuticle matter?