Synthesis of 8-bromo-, 8-methyl- and 8-phenyl-dATP and their polymerase incorporation into DNA
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18843392
DOI
10.1039/b811935j
Knihovny.cz E-resources
- MeSH
- Circular Dichroism MeSH
- Nucleic Acid Denaturation MeSH
- Deoxyadenine Nucleotides chemical synthesis chemistry metabolism MeSH
- DNA-Directed DNA Polymerase metabolism MeSH
- DNA genetics metabolism MeSH
- Base Sequence MeSH
- Transition Temperature MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 2'-deoxyadenosine triphosphate MeSH Browser
- Deoxyadenine Nucleotides MeSH
- DNA-Directed DNA Polymerase MeSH
- DNA MeSH
dATP derivatives bearing Br, Me or Ph groups in position 8 were prepared and tested as substrates for DNA polymerases to show that 8-Br-dATP and 8-Me-dATP were efficiently incorporated, while 8-Ph-dATP was a poor substrate due to its bulky Ph group.
References provided by Crossref.org
Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases
