Fatty acids, unusual glycophospholipids and DNA analyses of thermophilic bacteria isolated from hot springs
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Bacteria chemistry genetics isolation & purification MeSH
- DNA, Bacterial isolation & purification MeSH
- Species Specificity MeSH
- Phospholipids isolation & purification MeSH
- Glycolipids isolation & purification MeSH
- Fatty Acids isolation & purification MeSH
- Water Microbiology * MeSH
- Gas Chromatography-Mass Spectrometry MeSH
- Spectrometry, Mass, Fast Atom Bombardment MeSH
- Hot Temperature * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Phospholipids MeSH
- Glycolipids MeSH
- Fatty Acids MeSH
The composition of fatty acids in 12 strains of the genera Thermus, Meiothermus, Geobacillus and Alicyclobacillus was analyzed by gas chromatography-mass spectrometry. Major FAs found in the profiles included i-15:0, i-17:0, ai-15:0, i-16:0, 16:0, ai-17:0, together with some minor components. Branched FAs were predominant, forming more than 80% of all FAs measured. Fast atom bombardment-mass spectrometry was used for analysis of unusual glycophospholipids, i.e., acylglycosylcardiolipins from genera Geobacillus and Alicyclobacillus and 1-(hydroxy(2-(O-acylglycosyl-oxy)hexadecyloxy)phosphoryloxy) hexadecan-2-yl esters of C15-C17 acids from genera Thermus and Meiothermus. Cloning and preliminary sequence analysis of 16S rDNA showed that these isolates belong to the genera Thermus, Meiothermus, Geobacillus and Alicyclobacillus.
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