Plant antigens cross-react with rat polyclonal antibodies against KLH-conjugated peptides
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18992832
DOI
10.1016/j.cellbi.2008.10.003
PII: S1065-6995(08)00595-7
Knihovny.cz E-resources
- MeSH
- Antigens, Plant immunology metabolism MeSH
- Hemocyanins immunology MeSH
- Rats MeSH
- Molecular Sequence Data MeSH
- Peptides immunology MeSH
- Antibodies immunology metabolism MeSH
- Amino Acid Sequence MeSH
- Cross Reactions MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Plant MeSH
- Hemocyanins MeSH
- keyhole-limpet hemocyanin MeSH Browser
- Peptides MeSH
- Antibodies MeSH
Keyhole limpet hemocyanin (KLH)-conjugated peptides are routinely used to raise polyclonal antibodies for biochemical or immunolocalization studies. Rats are suitable for producing antisera against plant antigens as they often lack non-specific response towards plant materials. We attempted to obtain rat antisera against peptides derived from several plant proteins. However, most antisera recognized the same background KLH-related plant antigen (KRAP) in Arabidopsis and tobacco. We characterized KRAP with respect to size and cellular localization and examined possible antigen-specific reasons for the failure of most immunizations. We also found no reports of successful use of rat anti-KLH-peptide antibodies in plant studies. We thus believe that the rat-KLH:peptide system is poorly suited for production of antibodies, especially against plant antigens, and should be used with caution, if at all.
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