The structure of the small laccase from Streptomyces coelicolor reveals a link between laccases and nitrite reductases
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
G0500367
Medical Research Council - United Kingdom
PubMed
19063896
DOI
10.1016/j.jmb.2008.11.024
PII: S0022-2836(08)01461-7
Knihovny.cz E-resources
- MeSH
- Ascorbate Oxidase chemistry MeSH
- Ceruloplasmin chemistry MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Protein Structure, Quaternary MeSH
- Laccase chemistry MeSH
- Copper metabolism MeSH
- Models, Molecular MeSH
- Molecular Sequence Data MeSH
- Protein Multimerization MeSH
- Mutation genetics MeSH
- Nitrite Reductases chemistry metabolism MeSH
- Solutions MeSH
- Protein Structure, Secondary MeSH
- Amino Acid Sequence MeSH
- Sequence Alignment MeSH
- Protein Stability MeSH
- Streptomyces coelicolor enzymology MeSH
- Substrate Specificity MeSH
- Binding Sites MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Ascorbate Oxidase MeSH
- Ceruloplasmin MeSH
- Laccase MeSH
- Copper MeSH
- Nitrite Reductases MeSH
- Solutions MeSH
The X-ray structure of the two-domain laccase (small laccase) from Streptomyces coelicolor A3(2) was solved at 2.7-A resolution. The enzyme differs significantly from all laccases studied structurally so far. It consists of two domains and forms trimers and hence resembles the quaternary structure of nitrite reductases or ceruloplasmins more than that of large laccases. There are three trinuclear copper clusters in the enzyme localized between domains 1 and 2 of each pair of neighbor chains. In this way, a similar geometry of the active site as seen in large laccases is ensured, albeit by different arrangements of domains and protein chains. Three copper ions of type 1 lie close to one another near the surface of the central part of the trimer, and, effectively, a trimeric substrate binding site is formed in their vicinity.
References provided by Crossref.org
Yeast Hosts for the Production of Recombinant Laccases: A Review
Protein surface shielding agents in protein crystallization
PDB
3CG8
