Electrospun gelatin and poly-ε-caprolactone (PCL) nanofibers were prepared using needleless technology and their biocompatibility and therapeutic efficacy have been characterized in vitro in cell cultures and in an experimental model of a skin wound. Human dermal fibroblasts, keratinocytes and mesenchymal stem cells seeded on the nanofibers revealed that both nanofibers promoted cell adhesion and proliferation. The effect of nanofibers on wound healing was examined using a full thickness wound model in rats and compared with a standard control treatment with gauze. Significantly faster wound closure was found with gelatin after 5 and 10 days of treatment, but no enhancement with PCL nanofibers was observed. Histological analysis revealed enhanced epithelialisation, increased depth of granulation tissue and increased density of myofibroblasts in the wound area with gelatin nanofibers. The results show that gelatin nanofibers produced by needleless technology accelerate wound healing and may be suitable as a scaffold for cell transfer and skin regeneration.

Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague Czech Republic

Zobrazit více v PubMed

Minim Invasive Ther Allied Technol. 2010 Jun;19(3):144-56 PubMed

Tissue Eng Part A. 2009 Sep;15(9):2617-24 PubMed

Diabetes Care. 2003 Jun;26(6):1701-5 PubMed

J Surg Res. 2010 Jan;158(1):162-70 PubMed

Biomed Mater Eng. 2006;16(4 Suppl):S63-71 PubMed

PLoS One. 2008 Apr 02;3(4):e1886 PubMed

Langmuir. 2008 Mar 4;24(5):2051-6 PubMed

Int J Nanomedicine. 2006;1(1):15-30 PubMed

J Vasc Surg. 2005 May;41(5):837-43 PubMed

Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010 Sep-Oct;2(5):510-25 PubMed

Tissue Eng Part A. 2010 Nov;16(11):3527-36 PubMed

Biotechnol Adv. 2010 Jan-Feb;28(1):142-50 PubMed

J Mater Sci Mater Med. 2008 Sep;19(9):3093-104 PubMed

Int J Low Extrem Wounds. 2006 Mar;5(1):27-34 PubMed

Artif Organs. 2007 Nov;31(11):801-8 PubMed

Br J Dermatol. 2005 Jul;153(1):29-36 PubMed

J Nanosci Nanotechnol. 2006 Sep-Oct;6(9-10):2591-607 PubMed

Biomaterials. 2008 Feb;29(5):587-96 PubMed

J Invest Dermatol. 2007 May;127(5):998-1008 PubMed

Acta Biomater. 2008 Sep;4(5):1275-87 PubMed

J Biomed Mater Res B Appl Biomater. 2010 Feb;92(2):568-76 PubMed

Cytotherapy. 2009;11(7):874-85 PubMed

Wound Repair Regen. 2010 Sep-Oct;18(5):506-13 PubMed

Acta Biomater. 2009 Jul;5(6):1926-36 PubMed

J Immunol. 2008 Feb 15;180(4):2581-7 PubMed

J Biomed Mater Res A. 2010 Aug;94(2):499-508 PubMed

Biomaterials. 2010 May;31(13):3536-42 PubMed

Int Rev Cytol. 2007;257:143-79 PubMed

J Am Chem Soc. 2003 Jun 18;125(24):7146-7 PubMed

Recent Pat Nanotechnol. 2009;3(1):21-31 PubMed

Biomaterials. 2008 May;29(15):2293-305 PubMed

Biomed Microdevices. 2010 Aug;12(4):575-87 PubMed

J Drug Target. 2009 Nov;17(9):724-9 PubMed

J Pharm Sci. 2008 Aug;97(8):2892-923 PubMed

Biomed Mater. 2009 Aug;4(4):044106 PubMed

Opt Express. 2008 Jul 7;16(14):10815-22 PubMed

J Biomed Mater Res B Appl Biomater. 2004 Aug 15;70(2):286-96 PubMed

Biomed Mater. 2011 Feb;6(1):015001 PubMed

Biomaterials. 2006 Mar;27(8):1452-61 PubMed

Cell Transplant. 2010;19(10):1281-90 PubMed

Cyclosporine A Loaded Electrospun Poly(D,L-Lactic Acid)/Poly(Ethylene Glycol) Nanofibers: Drug Carriers Utilizable in Local Immunosuppression

A Comparative Study of the Therapeutic Potential of Mesenchymal Stem Cells and Limbal Epithelial Stem Cells for Ocular Surface Reconstruction

Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses