Real time RT-PCR with a newly designed set of primers confirmed the presence of 2b and 2x/d myosin heavy chain mRNAs in the rat slow soleus muscle
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
19154088
DOI
10.33549/physiolres.931676
PII: 1676
Knihovny.cz E-zdroje
- MeSH
- DNA primery * MeSH
- kosterní svaly chemie MeSH
- krysa rodu Rattus MeSH
- messenger RNA analýza MeSH
- polymerázová řetězová reakce * MeSH
- potkani inbrední LEW MeSH
- protein - isoformy MeSH
- svalová vlákna typu I chemie MeSH
- svalová vlákna typu II chemie MeSH
- těžké řetězce myosinu genetika MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA primery * MeSH
- messenger RNA MeSH
- protein - isoformy MeSH
- těžké řetězce myosinu MeSH
In order to re-evaluate the presence and relative quantity of 2b and 2x/d myosin heavy chain (MyHC) transcripts in rat slow soleus muscle by using real time RT-PCR we have compared the available relevant cDNA sequences and designed a new set of primers having similar melting temperatures, matching separate MyHC exons in the regions of maximal differences in MyHC coding sequences, and containing G or C at the 3 -end. These also yielded PCR products of corresponding length, which is an important requirement for real time RT-PCR quantification. The experiments were performed on 8-month-old inbred female Lewis strain rats used in our current study of regenerating transplanted muscles. The real time RT-PCR measurement confirmed the expression of all four MyHC mRNAs (type 1, 2a, 2x/d and 2b) in both fast extensor digitorum longus and slow soleus muscles, although in the soleus muscle of adult rats, only type 1 and 2a protein isoforms can be usually detected.
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