H3K9 acetylation and radial chromatin positioning
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19248079
DOI
10.1002/jcp.21734
Knihovny.cz E-resources
- MeSH
- Acetylation MeSH
- Adenocarcinoma enzymology genetics MeSH
- Cell Differentiation MeSH
- Cell Nucleus drug effects enzymology pathology MeSH
- HT29 Cells MeSH
- Butyrates pharmacology MeSH
- Cyclin D1 genetics MeSH
- Enterocytes drug effects enzymology pathology MeSH
- Epigenesis, Genetic * drug effects MeSH
- Genes, APC MeSH
- Histone Deacetylases metabolism MeSH
- Histones metabolism MeSH
- Enzyme Inhibitors pharmacology MeSH
- Histone Deacetylase Inhibitors MeSH
- Humans MeSH
- Chromosomes, Human, Pair 17 metabolism MeSH
- Chromosomes, Human, Pair 18 metabolism MeSH
- Chromosomes, Human, Pair 19 metabolism MeSH
- Chromosomes, Human metabolism MeSH
- Tumor Suppressor Protein p53 genetics MeSH
- Colonic Neoplasms enzymology genetics MeSH
- Protein Processing, Post-Translational * drug effects MeSH
- Cell Proliferation MeSH
- Promoter Regions, Genetic MeSH
- Gene Expression Regulation, Neoplastic MeSH
- Chromatin Assembly and Disassembly * drug effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Butyrates MeSH
- CCND1 protein, human MeSH Browser
- Cyclin D1 MeSH
- Histone Deacetylases MeSH
- Histones MeSH
- Enzyme Inhibitors MeSH
- Histone Deacetylase Inhibitors MeSH
- Tumor Suppressor Protein p53 MeSH
- TP53 protein, human MeSH Browser
Histone variants and their epigenetic modifications determine genome function, particularly transcription. However, whether regulation of gene expression can be influenced by nuclear organization or vice versa is not completely clear. Here, we analyzed the effect of epigenetic changes induced by a histone deacetylase inhibitor (HDACi) on the nuclear radial rearrangement of select genomic regions and chromosomes. The HDACi, sodium butyrate (NaBt), induced differentiation of human adenocarcinoma HT29 cells as well as a genome-wide increase in H3K9 acetylation. Three-dimensional analysis of nuclear radial distributions revealed that this increase in H3K9 acetylation was often associated with a repositioning of select loci and chromosomes toward the nuclear center. On the other hand, many centromeres resided sites more toward the nuclear periphery, similar to sites occupied by chromosome X. In more than two-thirds of events analyzed, central nuclear positioning correlated with a high level of H3K9 acetylation, while more peripheral positioning within interphase nuclei correlated with a lower level of acetylation. This was observed for the gene-rich chromosomes 17 and 19, TP53, and CCND1 genes as well as for gene-poor chromosome 18, APC gene, regions of low transcriptional activity (anti-RIDGEs), and the relatively transcriptionally less active chromosome X. These results are consistent with a role for epigenetic histone modifications in governing the nuclear radial positioning of genomic regions during differentiation.
References provided by Crossref.org
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SUV39h-independent association of HP1 beta with fibrillarin-positive nucleolar regions
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