Discrimination and genetic diversity of Wheat dwarf virus in the Czech Republic
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- DNA Fingerprinting MeSH
- DNA, Viral chemistry genetics MeSH
- Phylogeny MeSH
- Geminiviridae classification genetics isolation & purification MeSH
- Genetic Variation * MeSH
- Genotype MeSH
- Hordeum virology MeSH
- Molecular Sequence Data MeSH
- Plant Diseases virology MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Triticum virology MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- DNA, Viral MeSH
Cereal-infecting Mastreviruses are one of the most ubiquitous of viruses, having caused huge yield losses during the last decade in the Czech Republic. The presence of two strains of Wheat dwarf virus (WDV), one of which is wheat adapted and one barley adapted, have been confirmed from among field samples of wheat and barley plants. The virus typing was conducted by both PCR-RFLP and sequencing-based methods. The Czech WDV isolates of the barley strain are more variable than the isolates of the wheat strain, separating them into two clades, one representing a pool of divergent isolates. The RFLP analysis confirmed the separation of the Czech isolates into two strains and highlights a powerful method for the rapid diagnosis of both the wheat and barley strains. Additionally, both RFLP and sequence analysis have shown that the barley strain is restricted to the barley host, while the wheat strain is present in both wheat and barley plants.
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GENBANK
FJ546176, FJ546177, FJ546178, FJ546179, FJ546180, FJ546181, FJ546182, FJ546183, FJ546184, FJ546185, FJ546186, FJ546187, FJ546188, FJ546189, FJ546190, FJ546191, FJ546192, FJ546193
