Discrimination and genetic diversity of Wheat dwarf virus in the Czech Republic
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- DNA fingerprinting MeSH
- DNA virů chemie genetika MeSH
- fylogeneze MeSH
- Geminiviridae klasifikace genetika izolace a purifikace MeSH
- genetická variace * MeSH
- genotyp MeSH
- ječmen (rod) virologie MeSH
- molekulární sekvence - údaje MeSH
- nemoci rostlin virologie MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- pšenice virologie MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- DNA virů MeSH
Cereal-infecting Mastreviruses are one of the most ubiquitous of viruses, having caused huge yield losses during the last decade in the Czech Republic. The presence of two strains of Wheat dwarf virus (WDV), one of which is wheat adapted and one barley adapted, have been confirmed from among field samples of wheat and barley plants. The virus typing was conducted by both PCR-RFLP and sequencing-based methods. The Czech WDV isolates of the barley strain are more variable than the isolates of the wheat strain, separating them into two clades, one representing a pool of divergent isolates. The RFLP analysis confirmed the separation of the Czech isolates into two strains and highlights a powerful method for the rapid diagnosis of both the wheat and barley strains. Additionally, both RFLP and sequence analysis have shown that the barley strain is restricted to the barley host, while the wheat strain is present in both wheat and barley plants.
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EMBO J. 1989 Feb;8(2):359-64 PubMed
Virus Res. 2007 Jul;127(1):61-70 PubMed
Syst Biol. 2003 Oct;52(5):696-704 PubMed
J Gen Virol. 1977 Mar;34(3):475-83 PubMed
Mol Biol Evol. 2007 Aug;24(8):1596-9 PubMed
Plant Dis. 2007 Jan;91(1):111 PubMed
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82 PubMed
Plant Mol Biol. 1988 Jan;11(1):35-43 PubMed
Mol Biol Evol. 1987 Jul;4(4):406-25 PubMed
Arch Virol. 2002;147(1):205-16 PubMed
Bioinformatics. 1998;14(9):817-8 PubMed
Mol Biol Evol. 1993 May;10(3):512-26 PubMed
Nucleic Acids Res. 1990 Dec 25;18(24):7259-65 PubMed
Plant Dis. 2005 Aug;89(8):912 PubMed
Virus Genes. 2007 Jun;34(3):359-66 PubMed
GENBANK
FJ546176, FJ546177, FJ546178, FJ546179, FJ546180, FJ546181, FJ546182, FJ546183, FJ546184, FJ546185, FJ546186, FJ546187, FJ546188, FJ546189, FJ546190, FJ546191, FJ546192, FJ546193
