Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
- MeSH
- Geminiviridae klasifikace genetika izolace a purifikace MeSH
- ječmen (rod) virologie MeSH
- kvantitativní polymerázová řetězová reakce metody MeSH
- nemoci rostlin virologie MeSH
- pšenice virologie MeSH
- senzitivita a specificita MeSH
- virová nálož metody MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
A rapid method for detection, discrimination and quantification of wheat and barley strains of wheat dwarf virus (WDV) was successfully developed. The sensitivity of quantification of the wheat and barley strains of WDV ranged from an average of 1.2 × 10(7)-1.2 × 10(2) and from an average of 1.4 × 10(7)-1.4 × 10(4) copies of viral genome, respectively. These standard serial dilutions were applied to plant and vector tissues for virus titer calculations. Both strains of WDV were clearly discriminated by specific probes and melting curve analysis. Both TaqMan(®) and SYBR(®) Green technologies provided accurate and reliable methods for monitoring, detection, discrimination, and quantification of WDV.
Virus Genes. 2012 Dec;45(3):614 PubMed
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