Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus
Language English Country United States Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Geminiviridae classification genetics isolation & purification MeSH
- Hordeum virology MeSH
- Real-Time Polymerase Chain Reaction methods MeSH
- Plant Diseases virology MeSH
- Triticum virology MeSH
- Sensitivity and Specificity MeSH
- Viral Load methods MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
A rapid method for detection, discrimination and quantification of wheat and barley strains of wheat dwarf virus (WDV) was successfully developed. The sensitivity of quantification of the wheat and barley strains of WDV ranged from an average of 1.2 × 10(7)-1.2 × 10(2) and from an average of 1.4 × 10(7)-1.4 × 10(4) copies of viral genome, respectively. These standard serial dilutions were applied to plant and vector tissues for virus titer calculations. Both strains of WDV were clearly discriminated by specific probes and melting curve analysis. Both TaqMan(®) and SYBR(®) Green technologies provided accurate and reliable methods for monitoring, detection, discrimination, and quantification of WDV.
Virus Genes. 2012 Dec;45(3):614 PubMed
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