OBJECTIVE: Adipose tissue-derived factors represent important players in the metabolic regulations acting both on systemic and local level. However, their local concentrations in human adipose tissue are poorly described. METHODS: We measured 24-hour profile and post-glucose load concentrations of selected adipokines in the subcutaneous adipose tissue of 17 healthy women by in vivo microdialysis. During 24-hour period, subjects consumed two standardized meals (at 13.00 h and at 19.00 h). RESULTS: During 24-hour period, fat interleukin-6, interleukin-8/CXCL8, resistin, and hepatocyte growth factor (HGF) exhibited increase/decrease/plateau pattern and peaked at about 14.30 h. Fat leptin exhibited increase/plateau/decrease/increase pattern and reached plateau between 22.00 and 5.30 h. Fat adiponectin exhibited decrease/plateau pattern and reached plateau between 1.00 and 7.00 h. Fat plasminogen activator inhibitor-1 (PAI-1) exhibited decrease/increase pattern with the lowest value at 20.30 h. Oral glucose consumption significantly increased fat adiponectin and resistin levels and decreased fat leptin and PAI-1 levels, respectively. CONCLUSIONS: The levels of studied adipokines in subcutaneous fat exhibited significant variations during the 24-hour period after microdialysis catheter insertion that were not reflected in the circulation. Concentrations of adiponectin, resistin, leptin and PAI-1 were regulated by oral glucose ingestion from 1 to 3 h after oral glucose load in healthy women.

3rd Department of Medicine 1st Faculty of Medicine Charles University and General University Hospital U Nemocnice 1 128 00 Prague 2 Czech Republic

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