Re-differentiation of human articular chondrocytes is not enhanced by a rotary bioreactor system
Language English Country Czech Republic Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
19393130
PII: file/6115/fb2008a0030.pdf
Knihovny.cz E-resources
- MeSH
- Aggrecans metabolism MeSH
- Bioreactors MeSH
- Cell Differentiation * genetics MeSH
- Cell Culture Techniques MeSH
- Chondrocytes cytology metabolism transplantation MeSH
- Gene Expression MeSH
- Cartilage, Articular cytology metabolism MeSH
- Collagen Type II metabolism MeSH
- Collagen Type I metabolism MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Hydrogel, Polyethylene Glycol Dimethacrylate MeSH
- Tissue Engineering methods MeSH
- Tissue Scaffolds MeSH
- Versicans metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Aggrecans MeSH
- Collagen Type II MeSH
- Collagen Type I MeSH
- Hydrogel, Polyethylene Glycol Dimethacrylate MeSH
- Versicans MeSH
ACI is the most widely used cell-based surgical procedure for the repair of articular cartilage defects. The method is based on in vitro chondrocyte cultivation. Two different culture conditions, rotating-wall-vessel bioreactor and static culture, were assessed by their effect on the re-differentiation potential of human articular chondrocytes seeded into a hydrogel scaffold. Gene expression analysis of the tissue-engineered construct revealed no significant difference between the tested systems.
