The effect of DYS-14 copy number variations on extracellular fetal DNA quantification in maternal circulation
Language English Country United States Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
19456250
DOI
10.1089/dna.2009.0855
Knihovny.cz E-resources
- MeSH
- Child MeSH
- DNA blood MeSH
- Adult MeSH
- Gene Dosage * MeSH
- Gestational Age MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Chromosomes, Human, Y genetics MeSH
- Mothers MeSH
- Adolescent MeSH
- Young Adult MeSH
- Placental Insufficiency diagnosis genetics MeSH
- Fetus * MeSH
- Child, Preschool MeSH
- Prenatal Diagnosis * MeSH
- Sex-Determining Region Y Protein genetics MeSH
- Cell Cycle Proteins genetics MeSH
- Case-Control Studies MeSH
- Pregnancy MeSH
- Pregnancy Outcome MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Child, Preschool MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- DNA MeSH
- Sex-Determining Region Y Protein MeSH
- Cell Cycle Proteins MeSH
- SRY protein, human MeSH Browser
- TSPY1 protein, human MeSH Browser
The aims of our research involved to investigate DYS-14 copy number variations in healthy males, to quantify extracellular DNA in maternal circulation in normal versus complicated pregnancies, and to study variations in the DYS-14 copy number in extracellular male fetal DNA. Fifty-five healthy males, 43 uncomplicated male singleton pregnancies (23 sampled at the 16th week and 20 sampled at the 36th week), and 15 pregnancies with placental insufficiency (PI)-related complications (mean 34.1 weeks) were analyzed using real-time PCR with DYS-14 sequence, sex determining region Y (SRY), and beta-globin (GLO) genes used as markers. Increased levels of extracellular DNA were detected in PI-related complications relative to gestational age-matched controls (SRY, p < 0.001; DYS-14, p = 0.007; GLO, p < 0.001). When the mean + 2SD (standard deviation) of controls was used as a cutoff, SRY, DYS-14, and GLO achieved 91.7%, 68.8%, and 94.4% accuracy, respectively, for differentiation between normal and complicated pregnancies. Considerable variations in the DYS-14 copy number in healthy males (mean 52.6) and extracellular DNA were found. A lower DYS-14 copy number was observed in PI-related complications (mean 83.5) compared to uncomplicated pregnancies (16th week: mean 114.2, p = 0.02; 36th week: mean 142.8, p = 0.04). The DYS-14 copy number was higher in extracellular DNA throughout gestation relative to healthy males. We concluded that, regarding interindividual copy number variations, the DYS-14 sequence is not an optimal marker for extracellular fetal DNA quantification for differentiation between normal and complicated pregnancies.
References provided by Crossref.org
Circulating C19MC microRNAs in preeclampsia, gestational hypertension, and fetal growth restriction
