The objective of the study was to identify the profile of circulating C19MC microRNAs (miR-516-5p, miR-517*, miR-518b, miR-520a*, miR-520h, miR-525, and miR-526a) in patients with established preeclampsia (n = 63), fetal growth restriction (n = 27), and gestational hypertension (n = 23). We examined the correlation between plasmatic concentrations and expression levels of microRNAs and the severity of the disease with respect to clinical signs, requirements for the delivery, and Doppler ultrasound parameters. Using absolute and relative quantification approaches, increased extracellular C19MC microRNA levels (miR-516-5p, P = 0.037, P = 0.009; miR-517*, P = 0.033, P = 0.043; miR-520a*, P = 0.001, P = 0.009; miR-525, P = 0.026, P = 0.01; miR-526a, P = 0.03, P = 0.035) were detected in patients with preeclampsia. The association analysis pointed to no relationship between C19MC microRNA plasmatic concentrations and expression profile and identified risk factors for a poorer perinatal outcome. However, the dependence between the levels of plasmatic C19MC microRNAs and the pulsatility index in the middle cerebral artery and the values of cerebroplacental ratio was demonstrated. The study brought the interesting finding that the upregulation of miR-516-5p, miR-517*, miR-520a*, miR-525, and miR-526a is a characteristic phenomenon of established preeclampsia.

Clinic of Obstetrics and Gynecology 2nd Faculty of Medicine Charles University 5 Uvalu 84 150 06 Prague Czech Republic

Department of Molecular Biology and Cell Pathology 3rd Faculty of Medicine Charles University Ruska 87 100 00 Prague Czech Republic

Department of Molecular Biology and Cell Pathology 3rd Faculty of Medicine Charles University Ruska 87 100 00 Prague Czech Republic ; Institute for the Care of the Mother and Child 3rd Faculty of Medicine Charles University Podolske Nabrezi 157 36 147 00 Prague Czech Republic

Institute for the Care of the Mother and Child 3rd Faculty of Medicine Charles University Podolske Nabrezi 157 36 147 00 Prague Czech Republic

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The Prediction of Gestational Hypertension, Preeclampsia and Fetal Growth Restriction via the First Trimester Screening of Plasma Exosomal C19MC microRNAs

First trimester screening of circulating C19MC microRNAs and the evaluation of their potential to predict the onset of preeclampsia and IUGR

Expression profile of C19MC microRNAs in placental tissue in pregnancy-related complications

First trimester screening of circulating C19MC microRNAs can predict subsequent onset of gestational hypertension