Enhancing the resolution of the forward second harmonic imaging using the two-photon laser scanning microscope
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19500995
DOI
10.1016/j.micron.2009.04.007
PII: S0968-4328(09)00055-9
Knihovny.cz E-resources
- MeSH
- Lenses MeSH
- Microscopy, Fluorescence methods MeSH
- Photons MeSH
- Microscopy, Confocal methods MeSH
- Lasers MeSH
- Plant Leaves ultrastructure MeSH
- Bryopsida ultrastructure MeSH
- Microscopy, Polarization methods MeSH
- Thylakoids ultrastructure MeSH
- Image Enhancement MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
UNLABELLED: The effect of increasing the numerical aperture and changing the immersion imaging medium of the objective in the forward second harmonic imaging of the grana and intergranal parts of chloroplasts in the leaves of shade moss Plagiomnium affine plant were demonstrated. Also the enhancement of the forward second harmonic generation images and simultaneously records the forward and backward second harmonic signals were demonstrated. The second harmonic signals in the forward and backward directions are generated by a tightly focused linearly polarized femtosecond pulses laser. CONCLUSIONS: Forward second harmonic signals were successfully enhanced by capturing higher order of diffraction rays from the samples using high numerical aperture water immersion objective.
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