This study focuses on two representatives of experimentally uncharacterized haloalkane dehalogenases from the subfamily HLD-III. We report biochemical characterization of the expression products of haloalkane dehalogenase genes drbA from Rhodopirellula baltica SH1 and dmbC from Mycobacterium bovis 5033/66. The DrbA and DmbC enzymes show highly oligomeric structures and very low activities with typical substrates of haloalkane dehalogenases.

Institute of Experimental Biology and National Center for Biomolecular Research Masaryk University Brno Czech Republic

Zobrazit více v PubMed

Bosma, T., E. Kruizinga, E. J. de Bruin, G. J. Poelarends, and D. B. Janssen. 1999. Utilization of trihalogenated propanes by Agrobacterium radiobacter AD1 through heterologous expression of the haloalkane dehalogenase from Rhodococcus sp. strain m15-3. Appl. Environ. Microbiol. 65:4575-4581. PubMed PMC

Campbell, D. W., C. Muller, and K. F. Reardon. 2006. Development of a fiber optic enzymatic biosensor for 1,2-dichloroethane. Biotechnol. Lett. 28:883-887. PubMed

Chovancova, E., J. Kosinski, J. M. Bujnicki, and J. Damborsky. 2007. Phylogenetic analysis of haloalkane dehalogenases. Proteins 67:305-316. PubMed

Dravis, B. C., K. E. LeJeune, A. D. Hetro, and A. J. Russell. 2000. Enzymatic dehalogenation of gas phase substrates with haloalkane dehalogenase. Biotechnol. Bioeng. 69:235-241. PubMed

Egli, C., R. Scholtz, A. M. Cook, and T. Leisinger. 1987. Anaerobic dechlorination of tetrachloromethane and 1,2-dichloroethane to degradable products by pure cultures of Desulfobacterium sp. and Methanobacterium sp. FEMS Microbiol. Lett. 43:257-261.

Fetzner, S., and F. Lingens. 1994. Bacterial dehalogenases: biochemistry, genetics, and biotechnological applications. Microbiol. Rev. 58:641-685. PubMed PMC

Janssen, D. B. 2004. Evolving haloalkane dehalogenases. Curr. Opin. Chem. Biol. 8:150-159. PubMed

Janssen, D. B., J. Gerritse, J. Brackman, C. Kalk, D. Jager, and B. Witholt. 1988. Purification and characterization of a bacterial dehalogenase with activity toward halogenated alkanes, alcohols and ethers. Eur. J. Biochem. 171:67-72. PubMed

Jesenska, A., M. Bartos, V. Czernekova, I. Rychlik, I. Pavlik, and J. Damborsky. 2002. Cloning and expression of the haloalkane dehalogenase gene dhmA from Mycobacterium avium N85 and preliminary characterization of DhmA. Appl. Environ. Microbiol. 68:3724-3730. PubMed PMC

Jesenska, A., M. Pavlova, M. Strouhal, R. Chaloupkova, I. Tesinska, M. Monincova, Z. Prokop, M. Bartos, I. Pavlik, I. Rychlik, P. Mobius, Y. Nagata, and J. Damborsky. 2005. Mycobacterial haloalkane dehalogenases: cloning, biochemical properties, and distribution. Appl. Environ. Microbiol. 71:6736-6745. PubMed PMC

Keuning, S., D. B. Janssen, and B. Witholt. 1985. Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10. J. Bacteriol. 163:635-639. PubMed PMC

Krooshof, G. H., E. M. Kwant, J. Damborsky, J. Koca, and D. B. Janssen. 1997. Repositioning the catalytic triad aspartic acid of haloalkane dehalogenase: effects on stability, kinetics, and structure. Biochemistry 36:9571-9580. PubMed

Kulakova, A. N., K. A. Reid, M. J. Larkin, C. C. R. Allen, and L. A. Kulakov. 1996. Isolation of Rhodococcus rhodochrous NCIMB13064 derivatives with new biodegradative abilities. FEMS Microbiol. Lett. 145:227-231. PubMed

Kumari, R., S. Subudhi, M. Suar, G. Dhingra, V. Raina, C. Dogra, S. Lal, J. R. van der Meer, C. Holliger, and R. Lal. 2002. Cloning and characterization of lin genes responsible for the degradation of hexachlorocyclohexane isomers by Sphingomonas paucimobilis strain B90. Appl. Environ. Microbiol. 68:6021-6028. PubMed PMC

Monincova, M., Z. Prokop, J. Vevodova, Y. Nagata, and J. Damborsky. 2007. Weak activity of haloalkane dehalogenase LinB with 1,2,3-trichloropropane revealed by X-ray crystallography and microcalorimetry. Appl. Environ. Microbiol. 73:2005-2008. PubMed PMC

Nagata, Y., K. Miyauchi, J. Damborsky, K. Manova, A. Ansorgova, and M. Takagi. 1997. Purification and characterization of haloalkane dehalogenase of a new substrate class from a γ-hexachlorocyclohexane-degrading bacterium, Sphingomonas paucimobilis UT26. Appl. Environ. Microbiol. 63:3707-3710. PubMed PMC

Nagata, Y., Z. Prokop, Y. Sato, P. Jerabek, A. Kumar, Y. Ohtsubo, M. Tsuda, and J. Damborsky. 2005. Degradation of β-hexachlorocyclohexane by haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26. Appl. Environ. Microbiol. 71:2183-2185. PubMed PMC

Otyepka, M., and J. Damborsky. 2002. Functionally relevant motions of haloalkane dehalogenases occur in the specificity-modulating cap domains. Protein Sci. 11:1206-1217. PubMed PMC

Poelarends, G. J., M. Wilkens, M. J. Larkin, J. D. van Elsas, and D. B. Janssen. 1998. Degradation of 1,3-dichloropropene by Pseudomonas cichorii 170. Appl. Environ. Microbiol. 64:2931-2936. PubMed PMC

Ryoo, S. W., Y. K. Park, S. N. Park, Y. S. Shim, H. Liew, S. Kang, and G. H. Bai. 2007. Comparative proteomic analysis of virulent Korean Mycobacterium tuberculosis K-strain with other mycobacteria strain following infection of U-937 macrophage. J. Microbiol. 45:268-271. PubMed

Sato, Y., M. Monincova, R. Chaloupkova, Z. Prokop, Y. Ohtsubo, K. Minamisawa, M. Tsuda, J. Damborsky, and Y. Nagata. 2005. Two rhizobial strains, Mesorhizobium loti MAFF303099 and Bradyrhizobium japonicum USDA110, encode haloalkane dehalogenases with novel structures and substrate specificities. Appl. Environ. Microbiol. 71:4372-4379. PubMed PMC

Silberstein, M., J. Damborsky, and S. Vajda. 2007. Exploring the binding sites of the haloalkane dehalogenase DhlA from Xanthobacter autotrophicus GJ10. Biochemistry 46:9239-9249. PubMed

Stucki, G., and M. Thuer. 1995. Experiences of a large-scale application of 1,2-dichloroethane degrading microorganisms for groundwater treatment. Environ. Sci. Technol. 29:2339-2345. PubMed

Atypical homodimerization revealed by the structure of the (S)-enantioselective haloalkane dehalogenase DmmarA from Mycobacterium marinum

Multimeric structure of a subfamily III haloalkane dehalogenase-like enzyme solved by combination of cryo-EM and x-ray crystallography

A Haloalkane Dehalogenase from Saccharomonospora viridis Strain DSM 43017, a Compost Bacterium with Unusual Catalytic Residues, Unique (S)-Enantiopreference, and High Thermostability

Biochemical characterization of a novel haloalkane dehalogenase from a cold-adapted bacterium

Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58

Zobrazit více v PubMed

GENBANK
AM696288, AM696289