A comparison of HPLC/APCI-MS and MALDI-MS for characterising triacylglycerols in insects: species-specific composition of lipids in the fat bodies of bumblebee males
Language English Country Netherlands Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
19819198
DOI
10.1016/j.jchromb.2009.09.040
PII: S1570-0232(09)00685-0
Knihovny.cz E-resources
- MeSH
- Species Specificity MeSH
- Mass Spectrometry methods MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods MeSH
- Triglycerides chemistry MeSH
- Fat Body chemistry MeSH
- Bees chemistry MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Triglycerides MeSH
Two mass spectrometric methods for analysing triacylglycerols (HPLC/APCI-MS and MALDI-MS) were used and compared in terms of the relevance of the data for further biostatistical evaluation. While MALDI-MS is simpler and significantly faster, the time-consuming and labour-intensive HPLC/APCI-MS provides more complete information about the lipid components. However, both methods provide well-comparable results concerning the grouping of specimens belonging to different species when evaluated with multivariate exploratory approaches. The compositions of triacylglycerols in the fat bodies of males in 11 bumblebee species (Bombus terrestris, B. lucorum, B. lapidarius, B. pratorum, B. sylvarum, B. ruderatus, B. pomorum, B. subterraneus, B. campestris, B. bohemicus, and B. rupestris) were found to be species-specific.
References provided by Crossref.org
Fatty acids from pool lipids as possible precursors of the male marking pheromone in bumblebees
