A comparison of HPLC/APCI-MS and MALDI-MS for characterising triacylglycerols in insects: species-specific composition of lipids in the fat bodies of bumblebee males
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
19819198
DOI
10.1016/j.jchromb.2009.09.040
PII: S1570-0232(09)00685-0
Knihovny.cz E-zdroje
- MeSH
- druhová specificita MeSH
- hmotnostní spektrometrie metody MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody MeSH
- triglyceridy chemie MeSH
- tukové těleso chemie MeSH
- včely chemie MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- triglyceridy MeSH
Two mass spectrometric methods for analysing triacylglycerols (HPLC/APCI-MS and MALDI-MS) were used and compared in terms of the relevance of the data for further biostatistical evaluation. While MALDI-MS is simpler and significantly faster, the time-consuming and labour-intensive HPLC/APCI-MS provides more complete information about the lipid components. However, both methods provide well-comparable results concerning the grouping of specimens belonging to different species when evaluated with multivariate exploratory approaches. The compositions of triacylglycerols in the fat bodies of males in 11 bumblebee species (Bombus terrestris, B. lucorum, B. lapidarius, B. pratorum, B. sylvarum, B. ruderatus, B. pomorum, B. subterraneus, B. campestris, B. bohemicus, and B. rupestris) were found to be species-specific.
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Fatty acids from pool lipids as possible precursors of the male marking pheromone in bumblebees
