The age-dependent changes in the composition of triacylglycerols (TAG) in the fat bodies of bumblebee males were studied using HPLC/MS. Two related species (Bombus terrestris and B. lucorum) were compared, with the age of the males being 0-30 days. The total amount of TAG in B. lucorum was about 2.7 times higher than that in B. terrestris for all of the ages studied. One to three-day-old males had the highest content of TAG in their fat bodies (1.6-2.3 mg/individual in B. terrestris and 3.8-4.2 mg/individual in B. lucorum). The analytical data show different patterns in both species. The qualitative composition of fatty acids in TAG was similar, but the mean relative abundance between B. terrestris and B. lucorum differed: 14:0, 7 and 14%; 16:0, 20 and 44%; 18:3, 62 and 23%; 18:1, 3 and 8%, respectively (the data is based on a GC/MS integration). A statistical evaluation of the dynamic changes in the TAG composition revealed that in B. terrestris different age classes were well separated according to their TAG composition while in B. lucorum the TAG did not change substantially during the male's life. The TAG analyses provide more precise information on the differences between the classes studied than the FA composition alone.

Institute of Organic Chemistry and Biochemistry Academy of Sciences of the Czech Republic Flemingovo nám 2 166 10 Prague 6 Czech Republic

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Insect Biochem Mol Biol. 1999 Jun;29(6):481-514 PubMed

J Biol Chem. 1989 Oct 5;264(28):16335-8 PubMed

Lipids. 2008 May;43(5):441-50 PubMed

J Chromatogr A. 2006 Jan 6;1101(1-2):226-37 PubMed

Bioorg Med Chem. 1996 Mar;4(3):451-60 PubMed

J Chem Ecol. 2009 Jun;35(6):698-705 PubMed

Arch Insect Biochem Physiol. 2004 Sep;57(1):1-14 PubMed

Lipids. 1995 Apr;30(4):277-90 PubMed

Insect Biochem Mol Biol. 2001 Jan;31(1):7-17 PubMed

Annu Rev Entomol. 2010;55:207-25 PubMed

J Insect Physiol. 2008 Jan;54(1):204-14 PubMed

J Chromatogr A. 2010 Dec 24;1217(52):8186-94 PubMed

J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Nov 15;877(30):3878-84 PubMed

Rapid Commun Mass Spectrom. 2006;20(23):3586-94 PubMed

J Sep Sci. 2005 Aug;28(12):1315-33 PubMed

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