Analysis of triacylglycerols in fat body of bumblebees by chromatographic methods
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16246355
DOI
10.1016/j.chroma.2005.10.001
PII: S0021-9673(05)01921-7
Knihovny.cz E-resources
- MeSH
- Atmospheric Pressure MeSH
- Chromatography, Thin Layer methods MeSH
- Chromatography, Gas methods MeSH
- Triglycerides analysis MeSH
- Fat Body chemistry MeSH
- Bees MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Triglycerides MeSH
Triacylglycerols (TAGs) from the fat body of several bumblebee species (Bombus lucorum, B. terrestris, B. lapidarius, B. hypnorum, B. hortorum, and B. confusus) were studied using chromatographic techniques. Semi-preparative thin-layer chromatography was used to isolate the TAGs from the tissue extract. Gas chromatography (GC) enabled us to identify the fatty acids (FAs) that form bumblebee TAGs and to quantify their relative proportions. The TAGs were subsequently analysed by high-performance liquid chromatography-atmospheric pressure chemical ionisation mass spectrometry. Two chromatographic systems, including non-aqueous reversed-phase chromatography and silver ion chromatography on cation exchange resin in silver (I) ionic form, were optimised and their performance compared. The most abundant fatty acids in bumblebees TAGs contained 18 or 16 carbon atoms; oleic acid predominated in most samples. TAGs were found to be a complex mixture of isomers; some of them, e.g. OLnO, PLnO, PoPoO, PoPoP, POO, or OOO (where Po is palmitoleic, P is palmitic, Ln is linolenic, and O is oleic acid) were abundant in particular species. The composition of both FAs and TAGs was found to be species-specific. Only minor differences were found among specimens of the same species.
References provided by Crossref.org
Seasonal Dynamics in the Chemistry and Structure of the Fat Bodies of Bumblebee Queens
Fatty acids from pool lipids as possible precursors of the male marking pheromone in bumblebees
Non-polar lipid components of human cerumen
Unusual fatty acids in the fat body of the early nesting bumblebee, Bombus pratorum
