Nontargeted lipidomic characterization of porcine organs using hydrophilic interaction liquid chromatography and off-line two-dimensional liquid chromatography-electrospray ionization mass spectrometry
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Chromatography, Liquid methods MeSH
- Chromatography, Gas methods MeSH
- Phosphatidylethanolamines analysis MeSH
- Phospholipids analysis MeSH
- Spectrometry, Mass, Electrospray Ionization methods MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Liver chemistry MeSH
- Kidney chemistry MeSH
- Lipids analysis chemistry MeSH
- Fatty Acids analysis MeSH
- Spinal Cord chemistry MeSH
- Brain Chemistry MeSH
- Myocardium chemistry MeSH
- Plasmalogens analysis MeSH
- Lung chemistry MeSH
- Swine MeSH
- Spleen chemistry MeSH
- Stomach chemistry MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Phosphatidylethanolamines MeSH
- Phospholipids MeSH
- Lipids MeSH
- Fatty Acids MeSH
- Plasmalogens MeSH
Lipids form a significant part of animal organs and they are responsible for important biological functions, such as semi-permeability and fluidity of membranes, signaling activity, anti-inflammatory processes, etc. We have performed a comprehensive nontargeted lipidomic characterization of porcine brain, heart, kidney, liver, lung, spinal cord, spleen, and stomach using hydrophilic interaction liquid chromatography (HILIC) coupled to electrospray ionization mass spectrometry (ESI/MS) to describe the representation of individual lipid classes in these organs. Detailed information on identified lipid species inside classes are obtained based on relative abundances of deprotonated molecules [M-H](-) in the negative-ion ESI mass spectra, which provides important knowledge on phosphatidylethanolamines and their different forms of fatty acyl linkage (ethers and plasmalogens), phosphatidylinositols, and hexosylceramides containing nonhydroxy- and hydroxy-fatty acyls. The detailed analysis of identified lipid classes using reversed-phase liquid chromatography in the second dimension was performed for porcine brain to determine more than 160 individual lipid species containing attached fatty acyls of different acyl chain length, double-bond number, and positions on the glycerol skeleton. The fatty acid composition of porcine organs is determined by gas chromatography with flame ionization detection after the transesterification with sodium methoxide.
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