Bcr-Abl fusion sequences do not induce immune responses in mice when administered in mouse polyomavirus based virus-like particles
Language English Country Greece Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19885546
DOI
10.3892/ijo_00000441
Knihovny.cz E-resources
- MeSH
- Antigens, Viral immunology MeSH
- Fusion Proteins, bcr-abl immunology MeSH
- Leukemia, Myelogenous, Chronic, BCR-ABL Positive immunology MeSH
- Cytotoxicity, Immunologic MeSH
- Epitopes immunology MeSH
- Fluorescent Antibody Technique MeSH
- Microscopy, Immunoelectron MeSH
- Humans MeSH
- Mice MeSH
- Polyomavirus immunology MeSH
- Recombinant Proteins immunology MeSH
- Blotting, Western MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Viral MeSH
- Fusion Proteins, bcr-abl MeSH
- Epitopes MeSH
- Recombinant Proteins MeSH
Mouse polyomavirus-like particles (MPyV-VLPs) carrying inside a fragment of the Bcr-Abl hybrid protein containing the epitope of chronic myeloid leukemia fusion region were prepared. A sequence encoding 171 amino acids covering Bcr-Abl breakpoint was fused to the C-terminal part of VP3 minor protein connecting it to the VP1 capsomeres. Chimeric particles, the Bcr-Abl VLPs, were tested for their ability to induce Bcr-Abl specific immune response in mice after their intranasal (i.n.) or intraperitoneal (i.p.) administration without any other adjuvants. Bcr-Abl VLPs induced strong anti-VP1 immune response in both i.n. and i.p. immunized mice. As expected, neither IgG nor IgM anti-Bcr-Abl specific antibodies were detected in the sera of immunized animals. Surprisingly, no specific CTL (cytotoxic T-lymphocyte) activity was proved using two different methods (in vitro cytotoxicity assay with CFSE-labeled target cells and highly sensitive cytotoxicity assay using MHC class I Bcr-Abl specific pentamers). In addition, no proliferative response of T-cells of i.n. immunized mice after in vitro restimulation with antigen-pulsed bone marrow-derived dendritic cells was observed. Taken together, Bcr-Abl breakpoint epitopes appeared to be weak immunogens and even MPyV-VLPs did not provide sufficient adjuvant ability to support induction of immune responses specific to Bcr-Abl fusion zone epitope.
References provided by Crossref.org