The effect of new proteasome inhibitors, belactosin A and C, on protein metabolism in isolated rat skeletal muscle
Language English Country Spain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19886392
DOI
10.1007/bf03179064
Knihovny.cz E-resources
- MeSH
- Amino Acids metabolism MeSH
- Chymotrypsin antagonists & inhibitors metabolism MeSH
- Proteasome Inhibitors MeSH
- Cathepsin B metabolism MeSH
- Muscle, Skeletal drug effects metabolism MeSH
- Rats MeSH
- Intercellular Signaling Peptides and Proteins MeSH
- Peptides pharmacology MeSH
- Rats, Wistar MeSH
- Muscle Proteins metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amino Acids MeSH
- belactosin A MeSH Browser
- belactosin C MeSH Browser
- Chymotrypsin MeSH
- Proteasome Inhibitors MeSH
- Cathepsin B MeSH
- Intercellular Signaling Peptides and Proteins MeSH
- Peptides MeSH
- Muscle Proteins MeSH
The proteasome inhibitors are used as research tools to study of the ATP-dependent ubiquitin-proteasome system. Some of them are at present undergoing clinical trials to be used as therapeutic agents for cancer or inflammation. These diseases are often accompanied by muscle wasting. We herein demonstrate findings about new proteasome inhibitors, belactosin A and C, and their direct effect on protein metabolism in rat skeletal muscle. M. soleus (SOL) and m. extensor digitorum longus (EDL) were dissected from both legs of male rats (40-60 g) and incubated in a buffer containing belactosin A or C (30 microM) or no inhibitor. The release of amino acids into the medium was estimated using high performance liquid chromatography to calculate total and myofibrillar proteolysis. Chymotrypsin-like activity (CTLA) of proteasome and cathepsin B, L activity were determined by fluorometric assay. Protein synthesis and leucine oxidation were detected using specific activity of L-[1-14C] leucine added to medium. Inhibited and control muscles from the same rat were compared using paired t-test. The results indicate that after incubation with both belactosin A and C total proteolysis and CTLA of proteasome decreased while cathepsin B, L activity did not change in both SOL and EDL. Leucine oxidation was significantly enhanced in SOL, protein synthesis decreased in EDL. Myofibrillar proteolysis was reduced in both muscles in the presence of belactosin A only. In summary, belactosin A and C affected basic parameters of protein metabolism in rat skeletal muscle. The response was both muscle- and belactosin-type-dependent.
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Endocrinology. 2005 Jul;146(7):3141-9 PubMed
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J Nutr. 2000 Oct;130(10):2413-9 PubMed
Biochim Biophys Acta. 2004 Nov 29;1695(1-3):189-207 PubMed
Physiol Rev. 2002 Apr;82(2):373-428 PubMed
Nature. 1993 Sep 16;365(6443):264-7 PubMed
J Cell Physiol. 1999 Dec;181(3):455-61 PubMed
J Biol Chem. 1996 Oct 25;271(43):26690-7 PubMed
Annu Rev Immunol. 1999;17:739-79 PubMed
J Anim Sci. 1990 Aug;68(8):2362-70 PubMed
J Biol Chem. 1975 Jan 10;250(1):290-8 PubMed
Anal Biochem. 1980 May 15;104(2):284-9 PubMed
Org Lett. 2007 Apr 12;9(8):1537-40 PubMed
Eur J Clin Invest. 2005 Mar;35(3):157-63 PubMed
J Support Oncol. 2005 May-Jun;3(3):209-17 PubMed
Biochem J. 1977 Mar 15;162(3):557-68 PubMed
Clin Nutr. 2003 Oct;22(5):437-43 PubMed
J Chromatogr B Biomed Sci Appl. 1997 Aug 15;696(1):1-8 PubMed
Am J Physiol. 1999 Oct;277(4):E685-92 PubMed
J Clin Invest. 1996 Mar 15;97(6):1447-53 PubMed
Nature. 1991 Jan 10;349(6305):132-8 PubMed
Chembiochem. 2005 Feb;6(2):222-56 PubMed
Metabolism. 1986 Dec;35(12):1121-7 PubMed
Org Lett. 2004 Jun 24;6(13):2153-6 PubMed
Cancer Lett. 2002 Jun 6;180(1):69-74 PubMed
Nat Rev Mol Cell Biol. 2000 Nov;1(2):145-8 PubMed
J Clin Invest. 1997 Jul 1;100(1):197-203 PubMed
Clin Sci (Lond). 1998 Aug;95(2):225-33 PubMed
Biochem J. 1993 Apr 1;291 ( Pt 1):1-10 PubMed
Am J Physiol. 1998 Jan;274(1):R30-7 PubMed
Biochem Pharmacol. 2004 Jan 15;67(2):227-34 PubMed
Am J Physiol. 1999 Dec;277(6):E1077-86 PubMed
Int J Exp Pathol. 2004 Dec;85(6):365-71 PubMed
Physiol Res. 2005;54(5):541-7 PubMed
Anal Biochem. 1993 Jun;211(2):279-87 PubMed
J Biol Chem. 1951 Nov;193(1):265-75 PubMed
J Nutr. 1999 Jan;129(1S Suppl):227S-237S PubMed
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