Real-time monitoring of melittin-induced pore and tubule formation from supported lipid bilayers and its physiological relevance
Language English Country Ireland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19931234
DOI
10.1016/j.chemphyslip.2009.11.005
PII: S0009-3084(09)00355-7
Knihovny.cz E-resources
- MeSH
- Anti-Infective Agents chemistry pharmacology MeSH
- Membrane Fluidity drug effects MeSH
- Spectrometry, Fluorescence MeSH
- Lipid Bilayers chemistry MeSH
- Melitten chemistry pharmacology MeSH
- Microscopy, Atomic Force MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anti-Infective Agents MeSH
- Lipid Bilayers MeSH
- Melitten MeSH
The temporal evolution of effects of antimicrobial peptide melittin on supported phospholipid bilayers (SPBs) containing negatively charged phospholipids was monitored by ellipsometry and laser scanning microscopy together with measurements of lipid mobility by Z-scan fluorescence correlation spectroscopy. Under all conditions used in our study, we observed reproducibly two effects. The first one is formation of pores in the SPB, which occupy approximately 40% of the bilayer. The formation of pores was accompanied by a decrease in lateral diffusion coefficient of the lipids to approximately 60% of its initial value. The second, simultaneous, effect is the formation of tubules of approximately 30nm radius and length of the order of 10mum. Flushing of the sample with excess of buffer removes most of the tubules, but it does not affect the pores. Further experiments performed under various conditions demonstrated reproducibility of both phenomena.
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