GacS-dependent regulation of enzymic and antifungal activities and synthesis of N-acylhomoserine lactones in rhizospheric strain Pseudomonas chlororaphis 449
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
- MeSH
- antibióza * MeSH
- bakteriální proteiny genetika metabolismus MeSH
- houby fyziologie MeSH
- karboxylesterhydrolasy genetika metabolismus MeSH
- laktony metabolismus MeSH
- mutace MeSH
- polygalakturonasa genetika metabolismus MeSH
- Pseudomonas enzymologie genetika fyziologie MeSH
- půdní mikrobiologie * MeSH
- regulace genové exprese u bakterií MeSH
- transkripční faktory genetika metabolismus MeSH
- vývojová regulace genové exprese * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- bakteriální proteiny MeSH
- karboxylesterhydrolasy MeSH
- laktony MeSH
- pectinesterase MeSH Prohlížeč
- polygalakturonasa MeSH
- transkripční faktory MeSH
Pseudomonas chlororaphis strain 449 isolated from the rhizosphere of maize suppresses numerous plant pathogens in vitro. The strain produces phenazine antibiotics and synthesizes at least three types of quorum sensing signaling molecules, N-acylhomoserine lactones. Here we have shown that the rhizospheric P. chlororaphis strains 449, well known strain 30-84 as well as two other P. chlororaphis strains exhibit polygalacturonase activity. Using mini-Tn5 transposon mutagenesis, four independent mutants of strain P. chlororaphis 449 with insertion of mini-Tn5 Km2 in gene gacS of two-component GacA-GacS system of global regulation were selected. All these mutant strains were deficient in production of extracellular proteinase(s), phenazines, N-acylhomoserine lactones synthesis, and did not inhibit the growth of G(+) bacteria in comparison with the wild type strain. The P. chlororaphis 449-06 gacS (-) mutant studied in greater detail was deficient in polygalacturonase, pectin methylesterase activities, swarming motility and antifungal activity. It is the first time the involvement of GacA-GacS system in the regulation of enzymes of pectin metabolism, polygalacturonase and pectin methylesterase, was demonstrated in fluorescent pseudomonads.
Zobrazit více v PubMed
J Bacteriol. 2008 Apr;190(8):2759-66 PubMed
Appl Environ Microbiol. 1987 Sep;53(9):2237-41 PubMed
Nat Rev Microbiol. 2005 Apr;3(4):307-19 PubMed
J Bacteriol. 1988 Oct;170(10):4501-8 PubMed
Folia Microbiol (Praha). 2003;48(6):794-8 PubMed
J Bacteriol. 1990 Nov;172(11):6568-72 PubMed
FEMS Microbiol Rev. 2004 Jun;28(3):261-89 PubMed
Protein Expr Purif. 2007 Aug;54(2):300-8 PubMed
Microbiol Mol Biol Rev. 2005 Mar;69(1):155-94 PubMed
J Bacteriol. 1997 Dec;179(24):7663-70 PubMed
J Clin Microbiol. 1988 May;26(5):979-84 PubMed
Mol Plant Microbe Interact. 2006 Aug;19(8):924-30 PubMed
J Bacteriol. 1996 Nov;178(22):6618-22 PubMed
Microbiology (Reading). 1997 Dec;143 ( Pt 12):3703-3711 PubMed
Appl Environ Microbiol. 1999 Jun;65(6):2294-9 PubMed
Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6036-41 PubMed
Biochim Biophys Acta. 1960 Mar 11;38:460-9 PubMed
Mol Microbiol. 2008 Jan;67(2):241-53 PubMed
J Bacteriol. 1991 Jun;173(12):3814-20 PubMed
Trends Plant Sci. 2001 Sep;6(9):414-9 PubMed
Anal Biochem. 1976 May 7;72:248-54 PubMed
J Biol Chem. 1982 Jun 25;257(12):6669-76 PubMed
Appl Environ Microbiol. 2001 Sep;67(9):4305-15 PubMed
Appl Environ Microbiol. 1991 Mar;57(3):660-4 PubMed
Microbiology (Reading). 2006 Jan;152(Pt 1):43-58 PubMed
Methods Enzymol. 1994;235:386-405 PubMed
J Bacteriol. 2001 Jan;183(1):318-27 PubMed
Plant Mol Biol. 2001 Sep;47(1-2):311-40 PubMed
Genetika. 2008 Dec;44(12):1617-26 PubMed
Can J Microbiol. 2006 Dec;52(12):1177-88 PubMed
Eur J Biochem. 1975 Dec 15;60(2):349-55 PubMed
