Zamyslemí nad ,,postavenim" metody PCR v rutinní diagnostice toxoplazmózy pri vysetrení periferní krve u imunokompetentnich nemocných
[Considering the role of the PCR method in routine diagnosis of toxoplasmosis in peripheral blood tests in immunocompetent patients]
Language Czech Country Czech Republic Media print
Document type English Abstract, Journal Article
PubMed
19937577
- MeSH
- Child MeSH
- Adult MeSH
- Immunoglobulins blood MeSH
- Immunocompetence MeSH
- Complement Fixation Tests MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Polymerase Chain Reaction * MeSH
- Child, Preschool MeSH
- DNA, Protozoan blood MeSH
- Toxoplasma genetics isolation & purification MeSH
- Toxoplasmosis diagnosis immunology MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Child, Preschool MeSH
- Female MeSH
- Publication type
- English Abstract MeSH
- Journal Article MeSH
- Names of Substances
- Immunoglobulins MeSH
- DNA, Protozoan MeSH
Infections caused by pathogenic protozoan Toxoplasma gondii in our geographic area is the most frequent parasitic infection; Czech Republic declares seroprevalence approx. 30 %. Diagnosis of toxoplasmosis is mostly based on serological methods are used (EIA IgM, IgA, IgE, IgG and avidity in IgG, Western Blot, complement fixation). According to positive results of these tests diagnosis of acute toxoplasmosis is established. In our retrospective study we tried to evaluate results of T. gondii DNA positivity from blood samples by PCR compared with positive markers of acute infection in patients before specific therapy was initiated. In accordance with literature we concluded, that in routine examination of immunocompetent outpatients of Clinic of Infectious Diseases from the moment of 4 weeks after lymphatic nodes swelling protozoan DNA detection in blood sample is not possible.
