UNLABELLED: Expertomica Cells is a program for the creation and analysis of pedigree plots from time-lapse micrographs of cell monolayers. It enables recording the basic events in a cell cycle, cell neighbourhoods and spatial migration. The output is both numeric and graphical. The software helps to lower main hurdles in the manual analysis of cell monolayer development to practical limits; it reduces the operator processing time of typical experiment containing 5000 consecutive images from the usual 3 months to 3-10 h. AVAILABILITY AND IMPLEMENTATION: Freely available on the web at http://www.expertomicacells.tk or http://www.expertomicacells.wu.cz. The source code is implemented in JAVA 6 and supported by Linux, Mac and MS Windows. SUPPLEMENTARY INFORMATION: Supplementary data available at Bioinformatics online.

Institute of Physical Biology University of South Bohemia Academic and University Centre Zámek 136 373 33 Nové Hrady Czech Republic

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