Fluorescence lifetime tuning--a novel approach to study flip-flop kinetics in supported phospholipid bilayers
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- časové faktory MeSH
- chemické techniky analytické metody MeSH
- difuze MeSH
- fluorescence * MeSH
- fluorescenční barviva chemie MeSH
- fosfatidylcholiny chemie MeSH
- fosfatidylethanolaminy chemie MeSH
- fosfatidylseriny chemie MeSH
- fosfolipidy chemie MeSH
- kinetika MeSH
- lipidové dvojvrstvy chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 1,2-dielaidoylphosphatidylethanolamine MeSH Prohlížeč
- 1,2-dioleoylphosphatidylserine MeSH Prohlížeč
- 1,2-oleoylphosphatidylcholine MeSH Prohlížeč
- fluorescenční barviva MeSH
- fosfatidylcholiny MeSH
- fosfatidylethanolaminy MeSH
- fosfatidylseriny MeSH
- fosfolipidy MeSH
- lipidové dvojvrstvy MeSH
In the present work we introduce a straightforward fluorescent assay that can be applied in studies of the transbilayer movement (flip-flop) of fluorescent lipid analogues across supported phospholipid bilayers (SPBs). The assay is based on the distance dependent fluorescence quenching by light absorbing surfaces. Applied to SPBs this effect leads to strong differences in fluorescence lifetimes when the dye moves from the outer lipid leaflet to the leaflet in contact with the support. Herein, we present the basic principles of this novel approach, and comment on its advantages over the commonly used methods for investigating flip-flop dynamics across lipid bilayers. We test the assay on the fluorescent lipid analog Atto633-DOPE and the 3-hydroxyflavone F2N12S probe in SPBs composed of DOPC/ DOPS lipids. Moreover, we compare and discuss the flip-flop rates of the probes with respect to their lateral diffusion coefficients.
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