Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17042516
DOI
10.1021/la061934p
Knihovny.cz E-resources
- MeSH
- Time Factors MeSH
- Diffusion MeSH
- Phosphatidylcholines chemistry MeSH
- Lipid Bilayers chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1,2-oleoylphosphatidylcholine MeSH Browser
- Phosphatidylcholines MeSH
- Lipid Bilayers MeSH
The lateral diffusion coefficients of a BODIPY tail-labeled lipid in two model systems, namely, free-standing giant unilamellar vesicles (GUVs) and supported phospholipid bilayers (SPBs), were determined by fluorescence correlation spectroscopy (FCS) using the Z-scan approach. For the first time, the performed measurements on 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) bilayers maintain exactly the same experimental conditions for both systems, which allows for a quantitative comparison of lipid diffusion in these two commonly used model membranes. The results obtained revealed that the lipid mobility in free-standing bilayers (D=7.8+/-0.8 microm2 s-1) is significantly higher than in the bilayer created on the solid support (mica) (D=3.1+/-0.3 microm2 s-1).
References provided by Crossref.org
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