New horizons of genomic in situ hybridization
Language English Country Switzerland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
20110676
DOI
10.1159/000275796
PII: 000275796
Knihovny.cz E-resources
- MeSH
- Chromosomes, Plant * MeSH
- Phylogeny MeSH
- In Situ Hybridization methods MeSH
- Karyotyping MeSH
- Humans MeSH
- Base Sequence MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
This review summarizes conventional and recent applications of genomic in situ hybridization (GISH). GISH is a well recognized technique, but its modifications and applications have not been widely used. Here, we show how modifications to the GISH technique can be used as tools to 'paint' plant chromosomes. In addition, we describe novel applications, e.g. how GISH banding could be used for karyotyping plant chromosomes. We further discuss recent phylogenetic applications of GISH that allow a semiquantitative signal analysis and the possibility of comparing and combining this cytogenetic technique with DNA sequence-based phylogenetic trees.
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