Expression of alkaline proteinase gene in two recombinant Bacillus cereus feather-degrading strains
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- Bacillus cereus enzymologie metabolismus MeSH
- bakteriální proteiny biosyntéza MeSH
- bakteriální transformace MeSH
- endopeptidasy biosyntéza MeSH
- exprese genu * MeSH
- geny hub MeSH
- keratiny metabolismus MeSH
- kultivační média chemie MeSH
- kur domácí MeSH
- nestabilita genomu MeSH
- peří metabolismus MeSH
- plazmidy MeSH
- rekombinantní proteiny biosyntéza MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alkaline protease MeSH Prohlížeč
- bakteriální proteiny MeSH
- endopeptidasy MeSH
- keratiny MeSH
- kultivační média MeSH
- rekombinantní proteiny MeSH
Two Bacillus cereus feather-degrading strains (23/1 and 6/2) were transformed using a recombinant plasmid p5.2 carrying the alkaline proteinase gene (aprE). A high level of the aprE gene expression was observed when the recombinant strains were grown on sporulation medium. The expression of the aprE gene proceeded during the early stationary phase and the p5.2 plasmid was segregationally and structurally stable in both strains. The two recombinant strains grown on a mineral medium with 1 % chicken feather as source of energy, carbon and nitrogen exhibited higher proteolytic activity ( approximately 6-fold and 2.4-fold higher for strains 23/1 (p5.2) and 6/2 (p5.2), respectively. Keratinolytic activity increased approximately 3.5-fold and 4.15-fold, respectively. The keratinolytic activity further increased when an optimized medium with yeast extract and corn oil was used. Considerable amounts of free amino acids were obtained after the biodegradation of feather which makes the new strains promising for application in feather-waste treatment to, e.g., transformation to animal feedstuff.
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