Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
20405480
DOI
10.1002/jssc.200900809
Knihovny.cz E-zdroje
- MeSH
- antioxidancia chemie MeSH
- chemické modely MeSH
- chemické techniky analytické MeSH
- chromatografie micelární elektrokinetická kapilární metody MeSH
- Fagopyrum MeSH
- flavonoidy chemie MeSH
- glykosidy chemie MeSH
- koncentrace vodíkových iontů MeSH
- methanol chemie MeSH
- quercetin chemie MeSH
- rostlinné extrakty chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antioxidancia MeSH
- flavonoidy MeSH
- glykosidy MeSH
- methanol MeSH
- quercetin MeSH
- rostlinné extrakty MeSH
A simple and reliable method for determination of quercetin glycosides and free quercetin in buckwheat flower, leaves, stems and achenes was developed. The method consists of flavonoid extraction from freeze-dried homogenous material in 50% v/v methanol solution and in presence of an antioxidant, cleaning of extract and analyte isolation using SPE. Analytical step uses capillary micellar electrokinetic chromatography. The working ranges, LOD and LOQ, recovery, precision and measurement uncertainty were calculated. The method is suitable for samples from buckwheat. The highest content of rutin was found in flowers of both kinds of buckwheat (99,400 mg/kg in F. esculentum, 108,000 mg/kg in F. tataricum). The free quercetin occurs in flowers and achenes of F. esculentum, whereas flowers and achenes of F. tataricum contained quercitrin.
Citace poskytuje Crossref.org
